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Optimization of Process Parameters for Amylolytic Bacillus Subtilis LC060260 Isolated from Almora Region of Central Himalayas

Author Affiliations

  • 1Department of Biochemistry and Biochemical Engineering, Sam Higginbottam Institute of Agriculture and Sciences, Allahabad, 211007, India
  • 2Department of Biochemistry and Biochemical Engineering, Sam Higginbottam Institute of Agriculture and Sciences, Allahabad, 211007, India

Res. J. Engineering Sci., Volume 5, Issue (3), Pages 1-5, March,26 (2016)


Amylase producing bacteria were isolated from soil of Almora district of Uttarakhand, India. Twenty bacterial isolates were checked for their amylolytic activity on starch agar medium, strain that showed utmost zone of clearance was identified biochemically. Genomic analysis confirmed the strain as Bacillus subtilis. It was submitted to gene bank with accession LC060260. Different culture parameters were optimized for maximum alpha amylase activity and enhanced alpha amylase production 121.22 U/ml/min was observed under incubation period of 72 hr at 400C and medium pH 7.0.


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