Research Journal of Recent Sciences ________________________________________________ ISSN 2277 - 2502 Vol. 1(ISC-2011), 398-404 (2012) Res.J.Recent.Sci. Review Paper Initiation of Pharmaceutical Factories depending on more Application of Biotechnology on some Medicinal Plants Review Article (In Vitro Production of some Antioxidant, Analgesic, Antibacterial, Antidiabetic agents) Eman, A. Alam Botany Department, National Research Centre, Dokki, Giza, EGYPT Available online at, www.isca.in (Received 15th October 2011, revised 10th January 2012, accepted 25th January 2012) Abstract Higher plants are an important source of all type of substances, especially medicines (glycosides, ethereal oils, steroids, flavonoids, anthraquinones, alkaloids, tannins and saponins, etc.,). Traditionally the medicinal plants have been grown and then the active components extracted and this is likely to remain the normal procedure. However the production of medicinal plants can present problems, which have load to the search for other ways to produce naturally accruing substances: i. Production in the field is strongly dependent on season, weather, climate, diseases and pests. ii. Naturally occurring sources, especially in the tropics and subtropical zones, are becoming limited and some medicinal plants are extremely scarce. iiiThere may be technical and economic problems in production. iv. Production is labor intensive and therefore costs are high. v. There may be political instability in the country where the plants are available resulting in an interrupted supply. For the above mentioned reasons, attempts have been made to obtain substances from cell suspension cultures of higher plants, either through accumulation in the callus (biomass) or sometimes by the release into the nutrient medium. In this review we will discuss in vitro production of some antioxidant, analgesic, antibacterial, antidiabetic agents. Keywords: Glycosides, ethereal oils, steroids, flavonoids, weather, climate, production antibacterial, antidiabetic agents. Reference 1. Pierik R.LM., In vitro culture of higher plants, Martinus Nijhoff Publishers, Dordrecht, Netherland, (1987) 2. Dixon R.A. and Gonzales, Plant cell culture, Oxford University Press, Walton Street, Oxford, New York, USA. 2 nd Ed. (1994) 3. Matkowski A., Plant in vitro culture for the production of antioxidants – A review, Biotechnology Advances, 26, 548- 560 (2008) 4. Eman A. Alam, Gehan H. Amin, Yassin M. ElAyouty and Mohamed S. Abdel-Hady, Chemical composition and antibacterial activity studies on callus of Fagonia arabica L., Academia Arena, 2(12), 91-106 (2010) 5. Eman A. Alam, Cytological and Ultrastructural studies on callus of Fagonia arabica, New York Science Journal, 3(12), 154-157 (2010 a) 6. Eman A. Alam, Phytochemical screening on calli of Fagonia indica and Fagonia bruguieri Dc., New York Science Journal, 3(12), 158-164 (2010 b) 7. Eman A. Alam, In vitro production of callus from Zygophyllum coccineum L., Academia Arena, 3(1), 6- 9 (2011) 8. Aguirre Y.A.G., Zamilpa A., Cortazar M.G. and Tapia G.T., Adventitious root cultures of Castilleja tenuiflora Benth. as a source of phenylethanoid glycosides, Industrial Crops and Products, 36, 188– 195 (2012) 9. Georgiev M., Müller J.L., Weber J., Stancheva N. and Bley T., Bioactive metabolite production and stressrelated hormones in Devil’s claw cell sus-pension cultures grown in bioreactors, Applied Microbiology and Biotechnology, 89, 1683– 1691 (2011b) 10. Stancheva N., Weber J., Schulze J., Alipieva K., Müller J. L., Haas C., Georgiev V., Bley T. and Georgiev M., Phytochemical and flow cytometric analyses of Devil’s claw cell cultures, Plant Cell Tissue and Organ Culture, 105, 79–84 (2011) 11. Lubbe A. and Verpoorte R., Cultivation of medicinal and aromatic plants for specialty industrial materials, Industrial Crops and Products, 34, 785–801 (2011) 12. Sarasan V., Kite G.C., Sileshi G.W. and Stevenson P.C., Applications of phytochemical and in vitro techniques for reducing over-harvesting of medicinal and pesticidal plants and generating income for the rural poor, Plant Cell Reports, 30, 1163–1172 (2011)