Research Journal of Recent Sciences ________________________________________________ ISSN 2277 - 2502
Vol. 1(ISC-2011), 323-325 (2012)
Res.J.Recent.Sci.

Short Communication

Microbiological analysis of surface water in Indore, India
Sohani Smruti and Iqbal Sanjeeda
Department of Botany, Holkar Science College, Indore, INDIA

Available online at: www.isca.in
(Received 18th October 2011, revised7th January 2012 , accepted 24th January 2012)

Abstract
Water borne bacterial pathogens were isolated from surface waters in Indore, India. The bacterial pathogenic organisms can cause
intestinal infections, dysentery, typhoid fever, cholera, and other illnesses. The paper discusses results of bacteriological analysis
done on various water samples taken from different sources in Indore. The water samples collected from different regions of Indore
were found to have significant impurities, considerable deterioration and remarkable variation. In the bacteriological analysis,
colliform group of bacteria are differentiated by the presumptive test, confirmed test and completed test. After performing these
tests Gram negative, were non spore forming and rod shaped bacteria having metallic shine or pink with dark centerd colonies on
EMB agar indicated positive results. The isolates were characterized and identified as E.coli, Enterobacter, Klebsilla, salmonella
and Shigella. The study therefore, stresses on the need to control the fecal pollution of water bodies.
Keywords: E.coli, enterobacter, salmonella and shigella, presumptive test, eosin methylene blue (EMB) agar.

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