Research Journal of Recent Sciences _______________________________________________ ISSN 2277 - 2502
Vol. 1(ISC-2011), 317-319 (2012)
Res.J.Recent.Sci.

Short Communication

Biodiversity of Actinomycetes in Hypersaline soils of Kolhapur district and
their screening as potential antibiotic producer, India
Nakade D. B.
Dept. of microbiology, govt. Rajaram college,vidyanagar, Kolhapur, MS, INDIA

Available online at: www.isca.in
(Received 30th Sepatember 2011, revised 30th December 2011 , accepted 24th January 2012)

Abstract
Hyper saline Soil samples from different sites in Kolhapur district of Maharashtra were analysed over a period of one year for
actinomycetes population. Microscopic, cultural, biochemical characters, pigmentation, cell wall analysis and spore morphology
and other criteria were used to identify the Actinomycetes isolates. Members of genus Streptomyces were identified by MICRO-IS
software1. Isolates other than Streptomyces genus were identified using Bergeys manual of systematic bacteriologyy 2.The
actinomycetes species identified are Streptomyces (37),Streptoverticillum (20),Micromonospora (15),Nocardia (13),Microbispora
(08),Actinoplanes (5),Planomonospora(04),Kitasatosporia (3).Isolates were screened for antibacterial and antifungal activity by
Agar overlay technique using two bacterial and four fungal species.Results indicated that the hyper saline soils are rich in
biodiversity of actinomycetes, 4 species showed antifungal activity and 10 showed antibacterial activity.Present study indicated that
the halophillic actinomycetes have a great potential to be an antibiotic producer and can be explored for antibiotic production.
Keywords: Hyper saline soil, actinomycetes, agar overlay, screening, antibacterial, antifungal, biodiversity.

Reference
1. Richards L.A., Diagnosis and improvement of Saline
alkali soil, USDA Handbook No.60, Wasington, 69-82
(1954)
2. Kushner D.J., the Halobacteriaceae. In the Bacteria, a
treatise on structure and function, C.R. Woese and R.S.
Wolfe, Academic press, 8, 171-214 (1985)
3. Rodriguez Valera F., Characteristics and Microbial
ecology of Hyper saline environment, In Halophilic
Bacteria, (ed. F. Rodriguez-Valera, CRC press). Boca
Raton (Florida), III, 30, (1986)
4. Smit F.B., An investigation on a taint in ribbons of
bacon, the determination of halophillic Vibrios (n.sp.)
Proc.R.Coc.Queensland,49, 29-53 (1938)
5. Quesda E.A., Ventosa F., Rodriguez Valera And A.
Ramos-cormenza, Types and properties of some bacteria
isolated from hypersaline soils.J.Appl.Bacteriol, 53,155-
161 (1982)
6. Quesda E.A., Ventosa F., Rodriguez-valera , Megias I.
and Ramos-cormenza A., Numerical taxonomy of
moderately halophilic bacteria isolated from hypersaline
soils.J.Gen. Microbio, 129, 2649-57, (1983)
7. Quesda E., Valdermma M., Bejar V., VentosaA., Rulzb
erra quero F. And Ramos ormenza, A. Syst.Appl.
Microbiol, 9,132-137, (1987)
8. Collins C.H., Lyne P.M. and Granje J.M.,
Microbiological methods, Butterworth and Heinemann
pub, (1995)
9. Becker B., Lechevalier M.P. and Lechevalier H.A.,
Chemical composition of cell wall preparation from
strains of various form-genera of aerobic actinomycetes,
Appl.Microbiol, 13,236-243, (1965)