Research Journal of Recent Sciences _______________________________________________ ISSN 2277 - 2502
Vol. 1(ISC-2011), 313-316 (2012)
Res.J.Recent.Sci.

Short Communication

Levels of Glutathione S - Transferase In
Different Larval Tissues of Papilio Demoleus
Warade N. V.
Department of Zoology, Shankarlal Khandelwal College, Akola, M.S., INDIA

Available online at: www.isca.in
(Received 7th December 2011, revised 16th January 2012, accepted 28th January 2012)

Abstract
Papilio species are of economic importance, as their larvae are important defoliator of citrus known from almost all the citrus
growing areas of the world. As a result, the overall vigour and vitality of the citrus plant is reduced considerably. The aim of the
present article is to study the glutathione S-transferase in different larval tissues of Papilio demoleus. CDNB - conjugation
activity was compared in various fifth instar larval tissues of laboratory reared and field collected Papilio demoleus using CDNB
(1-chloro-2, 4-dinitrobenzene) as substrate. Different tissues estimated for GST include the midgut, fat bodies, cuticle,
haemolymph and the whole body of two days old fifth instar larvae. The GST activity was found highest (648.93 ± 0.08 µM mg
protein-1min-1) in fat bodies of field-collected larvae while the corresponding value in the laboratory-reared larvae was 80.19 ±
0.05 µM mg protein-1min-1, where it showed 8.09 fold increases in GST activity. The fat bodies showed highest GST activity
suggesting that the fat body is the major site of metabolism of insecticide, especially the organophosphate class, whiles the gut,
cuticle and haemolymph also plays an important role in the metabolism of insecticide.
Keywords: Glutathione S - transferase, Papilio demoleus, insecticide resistance, life cycle, GST

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