International Science Community Association Research Journal of Chemical Sciences 2231-606X 7 10 2017 10 18 Phytochemical analysis, antibacterial and antioxidant activities of Entada africana Guill. and perrott stem bark extracts 10 15 EN Subhi A. Al-Jibori 1 Chemistry Dept., College of Science, TikritUniversity, Tikrit, Iraq Mahmood M. Barbooti 2 Dept. Applied Science, University of Technology, P.O. Box 35045, Baghdad, Iraq Ahmed S.M. Al-Janabi 3 Dept Biochemistry, College of Veterinary Medicine, Tikrit University, Tikrit, Iraq A.H. Ali 4 Dept. Chemistry, College of Education, Ibnul-Haytham, University of Baghdad, Baghdad, Iraq N. Sami 5 Chemistry Dept., College of Science, TikritUniversity, Tikrit, Iraq Bakhtyar K. Aziz 6 Chemistry Department, University of Sulaimani, Sulaimani, Iraq S. Basak-Modi 7 Chemistry Dept., University College London, London WC1H 0AJ, UK A. Kwaji 1 Department of Chemistry, Faculty of Science, Gombe State University, Gombe, Nigeria H.M. Adamu 2 Department of Chemistry, Faculty of Science, Abubakar Tafawa Balewa University, Bauchi, Nigeria I.Y. Chindo 3 Department of Chemistry, Faculty of Science, Abubakar Tafawa Balewa University, Bauchi, Nigeria > Research Paper 2017 8 6 2017 10 18 Entada africana Guill and Perrot (Fabaceae) is predominantly a West African medicinal plant used in the treatment of several diseases. Multidrug resistance and the undesirable side effects of synthetic drugs underscore the need to search for alternatives from plants using bioassay guided technique. The hexane, dichloromethane, acetone and methanol stem bark extracts were screened for antibacterial activity. Relatively active acetone and methanol extracts were further analyzed for phytochemical contents and antioxidant property using standard methods. Results of the antibacterial screening indicates that the acetone extract had the best antibacterial property against Enterococcus faecalis, Escherichia coli and Staphylococcus aureus with inhibition zones ranging from 14.00�1.00 mm � 17.00�1.73 mm. Additionally, E. faecalis and E. coli had minimum inhibitory concentrations (MIC) of 0.39 mg/mL and 0.20 mg/mL respectively indicating moderate activity but weak on Staphylococcus aureus with MIC of 13.88 mg/mL. The quantitative phytochemical analysis of acetone and methanol extracts indicated 0.528�0.02 mg GAE/g and 0.650�0.85 mg GAE/g total polyphenol respectively. The acetone extract also yielded 0.500�0.017 mg RE/g of total flavonoids while the methanol extract gave 0.253�0.006 mg RE/g of total flavonoids. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) test for the crude extracts revealed similar antioxidant activity comparable to that of ascorbic acid standard and concentration dependent. The observed biological activities indicated the presence of bioactive phyto-constituents. The study therefore supports the therapeutic use of Entada africana as a herbal remedy in traditional medicine. Copyright@ International Science Community Association