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Assay Method Development and Validation of Biclotymolby High Performance Liquid Chromatography (HPLC)

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Author Affiliations

  • 1Department of Applied Science, Dr. K.N. Modi University, Newai, Rajasthan, India
  • 2Department of Applied Science, Dr. K.N. Modi University, Newai, Rajasthan, India
  • 3Department of chemistry, S.H.S Government P.G College, Dhanapur, Chandauli, India

Res.J.chem.sci., Volume 16, Issue (1), Pages 52-62, February,18 (2026)

Abstract

Biclotymol is a phenolic antiseptic API molecule which generally use for the treatment of mouth and throat infection. It is antibacterial, anti-inflammatory and analgesic. Due to phenolic antiseptic it kills bacteria, Biclotymol have fast acting and long lasting molecule. The Method Validation is applicable for demonstration of method accuracy, method precision, linearity, repeatability, reproducibility, limit of detection(LOD) and limit of quantitation(LOQ) for analysis of Biclotymol for assay. High Performance Liquid Chromatography(HPLC) equipped with VWD detector and stainless steel column 25 cm×4.6 mm, C18 (5µm). From the analytical method validations report, it is concluded that the Assay of Biclotymol is (96.0%- 101.0%).

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