International Science Community Association Research Journal of Chemical Sciences 2231-606X 14 2 2024 06 18 Simple and sensitive LC-ESI-MS method for estimation of dexketoprofen in plasma samples 12 18 EN DAZOGBO Pascal Gbétondji 1 Laboratory of Physical Chemistry, Materials and Molecular Modeling / Unit of Inorganic Chemistry, Materials Engineering and Environment (LCP3M / UCIIME), Faculty of Science and Technology (FAST), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benign and Laboratory of Water and Environmental Sciences and Techniques (LSTEE), National Water Institute (INE), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benin NONVIHO Guevara 2 Laboratory for Study and Research in Applied Chemistry (LERCA), Ecotoxicology and Quality Study Research Unit (UREEQ), Polytechnic School of Abomey-Calavi (EPAC/UAC), Cotonou, Benin OSSENI Sèmiyou Ayélé 3 Applied Hydrology Laboratory (LHA), National Water Institute (INE), University of Abomey-Calavi (UAC), 01BP526 Cotonou, Benin YESSOUFOU Arouna 4 Kaba Chemistry and Applications Research Laboratory, Faculty of Sciences and Technologies of Natitingou, UNSTIM, Benin ZANOU Philémon Mindétonhou 5 Laboratory of Physical Chemistry, Materials and Molecular Modeling / Unit of Inorganic Chemistry, Materials Engineering and Environment (LCP3M / UCIIME), Faculty of Science and Technology (FAST), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benign and Laboratory of Water and Environmental Sciences and Techniques (LSTEE), National Water Institute (INE), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benin GOUNDJO Ferdinand Sèdjro Didier 6 Laboratory of Physical Chemistry, Materials and Molecular Modeling / Unit of Inorganic Chemistry, Materials Engineering and Environment (LCP3M / UCIIME), Faculty of Science and Technology (FAST), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benign CHOUTI Waris Kéwouyèmi 7 Laboratory of Physical Chemistry, Materials and Molecular Modeling / Unit of Inorganic Chemistry, Materials Engineering and Environment (LCP3M / UCIIME), Faculty of Science and Technology (FAST), University of Abomey-Calavi (UAC), 01BP 526 Cotonou, Benign Zaware Sandeep 1 Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India Lahoti Neel 2 Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India Chaudhari Shubham 3 Synergen Bio Private Limited, Unit Nos. 101 to 104 and 309 to 311, Sai Chambers, 302, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India > Research Paper 2023 12 16 2024 06 18 A simple and sensitive LC-ESI-MS assay was developed to determine the concentration of dexketoprofen in human plasma. Dexketoprofen and ketoprofen D3 (internal standard) were extracted from the plasma using solid phase extraction technique and injected onto Phenomenex Luna C18 100mm*4.6 mm, 3µm. The mobile phase consisted of Acetonitrile: 0.2% Formic Acid (65:35, v/v) and was delivered isocratically at a flow rate of 1.0 mL/min (Split 50:50 v/v). The MRM mode for dexketoprofen and internal standard ketoprofen D3 were detected at m/z 255.1→209.2 and 258.1→212.2 and the run time was 3 min. The detection limit of dexketoprofen was 50.0ng/mL, and the calibration curve was linear between 50.0 and 6000.0ng/ml. Concentrations of drugs were determined by using a validated LC-MS/MS method. Copyright@ International Science Community Association