International Science Community Association Research Journal of Chemical Sciences 2231-606X 12 1 2022 02 18 Wound healing activity of Mangifera indica seed extracts in Wistar Rats 1 13 EN I.A. Ajayi 1 Industrial Unit, Chemistry Department, Faculty of Science, University of Ibadan, Nigeria O.B. Omolere 2 Industrial Unit, Chemistry Department, Faculty of Science, University of Ibadan, Nigeria > Research Paper 2020 9 25 2022 02 18 Mangifera indica is among the plants of high medicinal value in ethno medicine’s system in Nigeria that is used in treating many ailments. Different parts of M. indica plant have been widely used but the activity of its seed extracts for healing of wound has not been documented scientifically hence the need for precise information about this so as to be able to expand the use of the plant and thereby include its integration into modern medical healthcare systems. Present study investigated the activity of hexane and methanolic seed extracts of M. indica for healing of wounds using Wistar rats. Phytochemical analysis of the seed extracts was determined so as to know their various phytoconstituents. The extracts were examined for antimicrobial activity on multiple drug resistant bacteria (P. aeruginosa: PA, S. aureus: SA, B. subtilis: BS and E. coli: EA) and fungi (A. niger: AN and C. albicans: CA) using agar technique of pour plate and surface plate dilution respectively. The effect of M. indica seeds for healing of wound was evaluated by incorporating the hexane and methanol extracts into paraffin in 5% and 10% (v/v) concentrations. 35 Wistar rats which were distributed into 5 groups of n=7 in each group, were given water plus feed continually for the study that lasted for a period of 21 days. The weight, area of wound (mm2) and wound closure percentage were noted on a four-day basis with epitheliasation of each wound measured from the 16th to 20th day of study period. Blood samples with skin and tissues (liver, kidney, heart, lung and spleen) of animals from each group were then subjected to haematological and histopathological analyses respectively. Phytochemical analysis revealed the existence of various chemical constituents including tannins, glycosides plus phenols. M. indica methanol extract recorded MIC value of 12.5% against SA, BS, CS and PA while that of AN was 25%. MIC value for M. indica hexane extract was 12.5% against SA, 25% against BS, CA, EC and PA and 50% against AN. Group 3 rats that were treated with 5% (v/v) methanol extract ointment formulate of M. indica seeds had the fastest epithelialisation time with healing time of 16.74±0.18 days as compared with control rats (group 1) that had ointment for treatment. Almost all the test rats treated with both methanol and hexane extracts displayed epithelialisation times that were better and faster than those of the control rats. The control and test rats showed no significant differences for their histopathological and haematological analyses results. This is an indication that the extracts contain chemical constituent that accelerated the wound healing process. M. indica seed extract exhibited good efficacy for wound healing and might therefore serve as a good replacement for conventional ointment used in treatment of wound. Copyright@ International Science Community Association