International Science Community Association Research Journal of Chemical Sciences 2231-606X 11 3 2021 10 18 Chemical characterization of the coagulating solutions of the powders of the seeds of Arachis hypogaea L., Cucumeropsis mannii Naud. and Moringa oleifera Lam. 14 23 EN . Anupama 1 Dept. in Chemistry, Govt. Girls College, Jhunjhunu, Rajasthan, India Kumawat Pooja 2 Dept. in Chemistry, Govt. PG College Jhunjhunu, Rajasthan, India Sharma Kanika 1 Department of Chemistry, University of Rajasthan, Jaipur-302004, India Saxena Sanjiv 2 Department of Chemistry, University of Rajasthan, Jaipur-302004, India Jain Asha 3 Department of Chemistry, University of Rajasthan, Jaipur-302004, India NTALANI Hermeline 1 Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo and Institute for Research in Exact and Natural Sciences, BP 2400, Brazzaville, Congo NGOUANOU Ravelle Duclérine 2 Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo MAKOMO Hubert 3 Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo LOUMOUAMOU Aubin Nestor 4 Multidisciplinary Research Team in Food and Nutrition, Faculty of Sci. and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo and Institute for Research in Exact and Natural Sciences, BP 2400, Brazzaville, Congo MOULOUNGUI Zéphirin 5 Dina – BioRes# - Chem www.dinabiores.com 10 rue Simone Henry 31200 Toulouse, France OUAMBA Jean-Maurille 6 Plant and Life Chemistry Unit, Faculty of Sciences and Technics, University Marien NGOUABI, BP 69, Brazzaville, Congo > Research Paper 2021 4 2 2021 10 18 The aim of this study is to characterize the proteins in the coagulating solutions of the powders of the seeds of Arachis hypogaea L., Cucumeropsis mannii Naud. and Moringa oleifera Lam. Analyzes were performed using high performance size exclusion liquid chromatography (SE-HPLC) on a Superdex column in the range of 10kDa to 500kDa and on a Shodex column in the range of 204Da to 35000Da. Electrophoresis on polyacrylamide gel containing sodium dodecyl sulfate (SDS PAGE) was also performed. The results obtained showed that the coagulating solutions of C. mannii and M. oleifera mainly contain small proteins, of which 75.4% and 94.4% have molecular masses of less than 10kDa. The coagulating solution of A. hypogaea, on the other hand mainly contains large proteins, of which 25.4% have molecular masses between 100kDa and 300kDa, 16.8% between 300kDa and 500kDa and 16.8% have molecular masses greater than 500kDa. Copyright@ International Science Community Association