International Science Community Association Research Journal of Chemical Sciences 2231-606X 11 3 2021 10 18 Substance ingredient of Anisodus luridus roots and their antimicrobial transmission 1 5 EN . Anupama 1 Dept. in Chemistry, Govt. Girls College, Jhunjhunu, Rajasthan, India Kumawat Pooja 2 Dept. in Chemistry, Govt. PG College Jhunjhunu, Rajasthan, India > Research Paper 2021 2 17 2021 10 18 The feeble base and sturdy base fractions were separated from methanolic extract of roots of Anisodus luridus. Chromatographic resolution of both fractions yielded composite 1-5 which is characterized as a β-sitosteroal (1), β-sitosteroal -β-D-glucoside (2), apohyoscyamine (3), esculetin (4) and hyoscyamine (5) with the help out of spectroscopic analysis. Four compounds, 1-4 were isolated from A. luridus for the first time. The weak base, strong base and isolated ingredient were screened against six microbes viz. Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Candida albicans, Candida tropicalis with Candida krusei. feeble base, sturdy base and isolated pure compounds exhibited their potential against these microbes. Copyright@ International Science Community Association