International Science Community Association International Research Journal of Environmental Sciences 2319-1414 9 1 2020 01 22 Current trends in enzymatic biosensors for pesticides determination 87 107 EN E. Jayantakumar Singh 1 Department of Chemistry, Manipur University, Canchipur, Imphal, India N. Rajmuhon Singh 2 Department of Chemistry, Manipur University, Canchipur, Imphal, India Rahul K. Kamble 1 Centre for Higher Learning and Research in Environmental Science, Sardar Patel College, Ganj Ward, Chandrapur 442 402, India M.S. Rahman 1 Department of Environmental Science, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh M.A. Islam 2 Department of Environmental Science, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh M.S. Hossen 3 Department of Environmental Science, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh Kassaye Gurebiyaw 1 Department of Natural Resources Management, College of Agriculture and Environmental sciences, University of Gondar, Ethiopia Melese Yigzaw 2 Department of Natural Resources Management, College of Agriculture and Environmental sciences, University of Gondar, Ethiopia Hailu Kendie 3 Amhara Region Agricultural Research Institute (ARARI), Soil and Water Conservation Directorate, Bahir Dar, Ethiopia Gebretsadik Melak 4 Department of Natural Resources Management, College of Agriculture and Environmental sciences, University of Gondar, Ethiopia Mohammed Raffi1 5 Department of Natural Resources Management, College of Agriculture and Environmental sciences, University of Gondar, Ethiopia Alemtsehay Hagos 6 Department of Natural Resources Management, College of Agriculture and Environmental sciences, University of Gondar, Ethiopia M. Rezai 1 Department of Engineering, Ibn-e-Sina University, Kabul, Afghanistan Nihal Thakare 1 Department of Environmental Engineering, K.I.T.′s College of Engineering (Autonomous), Kolhapur, India Bharat Ingavale 2 Department of Environmental Engineering, K.I.T.′s College of Engineering (Autonomous), Kolhapur, India Noel Dube 1 Department of Geography and Environmental Studies, Zimbabwe Open University, Box 346, Gwanda, Zimbabwe Hussen Yasin 1 Departments of Biology, Werabe University, Ethiopia Biniam Asfaw 2 Departments of Biology, Wolaita Sodo University, Ethiopia Eyasu Chama 3 Departments of Biology, Wolaita Sodo University, Ethiopia Bisrat Kifle Arsiso 1 Ethiopian Civil Service University, College of Urban Development and Engineering, Department of Environment and Climate Change Management, Addis Ababa Ethiopia, P O Box 5648 Nimisha Tehri 1 Department of Microbiology, IIMT University, Meerut, UP, 250001, India and Microbial Biosensors & Food Safety Laboratory, DM Division, ICAR-NDRI, Karnal 132001, Haryana, India Naresh Kumar 2 Microbial Biosensors & Food Safety Laboratory, DM Division, ICAR-NDRI, Karnal 132001, Haryana, India Amit Vashishth 3 Department of Biotechnology, MMDU, Mullana, Ambala, Haryana, 133207, India > Review Paper 2019 6 18 2020 01 22 Owing to the documentation of worldwide surveys of pesticides in different food products and high mortality rate associated with its exposure to environment and human, pesticides has become a serious public health concern. Maximum residual limits for pesticides as a legal requirement have been laid down by several regulatory bodies throughout the world. It is very important to quantify pesticide residues by using different analytical methods which are extremely susceptible and accurate due to the trace level of pesticides. Although conventional analytical methods, based on different chromatographic techniques like GC, HPLC coupled with mass selective detectors, fulfil these requirements. Despite, these have intrinsic demerits e.g. limited scope of application under field conditions, time-consuming, cost-effective and are not ease for the direct analysis of pesticides residue in real samples. To address this issue, development of biosensors as rapid alternative techniques for pesticides determination is predominant goal. Enzyme based biosensors has become very popular for their sensitivity and high efficient analysis of pesticides over few past decades. The present article mainly demonstrates the recent advancement in the development of enzymatic biosensors for pesticides determination. Enzyme based biosensors have been classified according to their catalytic and inhibition mechanism for sensing of pesticides. Their construction, mode of immobilization and analytical characteristics are discussed. Applications in the field of environmental safety, food safety and future prospects for development of more superior enzyme based sensing technologies for pesticides determination are also delineated. Copyright@ International Science Community Association