International Science Community Association International Research Journal of Environmental Sciences 2319 - 1414 6 9 2017 09 22 Isolation and characterization of biosurfactant producing microorganisms from marine of Gujarat and their role in bioremediation 19 25 EN Peter Nabam 1 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Majumdar Jayjeet 2 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Biswas Gaurav 3 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Singh Harpreet 4 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Francis Nimmy 5 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Sengupta Anirrudha 6 Department of Civil Engineering, Indian Institute of Technology Kharagpur, West Bengal, India Mitra Anirban 7 Department of Computer Science and Engineering, Vignan Institute of Technology and Management, Berhampur, Odisha, India Mitra Analava 8 School of Medical Science and Technology, Indian Institute of Technology Kharagpur, West Bengal, India Singh E. Jayantakumar 1 Department of Chemistry, Manipur University, Canchipur, Imphal, India Singh N. Rajmuhon 2 Department of Chemistry, Manipur University, Canchipur, Imphal, India Gupta A. 3 Department of Environmental Sciences, Assam University, Silchar, India Khodabhai Patel Mehul 1 Department of Biochemistry, The Oxford College of Science, Bangalore–560102, Karnataka, India K.S. Bhanuprakash 2 Department of Biochemistry, The Oxford College of Science, Bangalore–560102, Karnataka, India Kalita Poonam 3 Department of Biochemistry, The Oxford College of Science, Bangalore–560102, Karnataka, India Bora Nikhita Nina 4 Department of Biochemistry, The Oxford College of Science, Bangalore–560102, Karnataka, India Roy Sangita 5 Department of Biochemistry, The Oxford College of Science, Bangalore–560102, Karnataka, India > Research Paper 2017 6 12 2017 09 22 Petroleum based hydrocarbon degrading and biosurfactant producing bacterial strains were isolated from Alang, ship breaking yard of Bhavnagar, Gujarat. Three strains were identified as Pseudomonas sp, Pseudomonas aeruginosa and Serratia marcescens on the basis of 16S rDNA sequencing analysis and Gene Bank submission data. The hemolytic activity, methylene blue agar plate method, drop collapsing test as well as oil displacement test were performed to elucidate biosurfactant producing activity of the bacterial strains. Foaming percentages of the crude biosurfactant were found to be 30.8% ± 0.58 to 43.8 % ± 0.43. All the strains were found to be efficiently degrading petroleum based oils and edible oils. Emulsification index against different hydrocarbons showed their diverse application in bioremediation. FTIR analysis revealed the presence of lipid and peptide in the isolated biosurfactants. Copyright@ International Science Community Association