International Science Community Association International Research Journal of Environmental Sciences 2319-1414 13 4 2024 10 22 Cytotoxicity study of the hospital liquid effluents and their chemical depollution by clays of the Benin: Case of CNHU-HKM of Cotonou, Benin 11 17 EN Pathak Jaishree Purohit 1 Institute of Sciences, Sage University, Indore, India Upadhyay Siya 2 Institute of Sciences, Sage University, Indore, India A.M. Kasimakhunova 1 Fergana Polytechnic Institute, Fergana, Uzbekistan M.A. Norbutaev 2 Fergana Polytechnic Institute, Fergana, Uzbekistan CAKPO Arthur Romuald 1 Laboratoire de Chimie Physique-Matériaux et Modélisation Moléculaire (LCP3M), Unité de recherche de Chimie Inorganique, Ingénierie des Matériaux et Environnement (URCIIME), Faculté des Sciences et Techniques (FAST), Université d’Abomey, Calavi (UAC). République du Bénin Cowiyou SEIDOU O. 2 Laboratoire de Chimie Physique-Matériaux et Modélisation Moléculaire (LCP3M), Unité de recherche de Chimie Inorganique, Ingénierie des Matériaux et Environnement (URCIIME), Faculté des Sciences et Techniques (FAST), Université d’Abomey, Calavi (UAC). République du Bénin Etienne SAGBO 3 Laboratoire de Chimie Physique-Matériaux et Modélisation Moléculaire (LCP3M), Unité de recherche de Chimie Inorganique, Ingénierie des Matériaux et Environnement (URCIIME), Faculté des Sciences et Techniques (FAST), Université d’Abomey, Calavi (UAC). République du Bénin > Research Paper 2024 7 17 2024 10 22 The chemical substances used in the hospitals for various activities are often found in the hospital liquid effluents. The rejection of these effluents in the natural environment is a health risk human, and can cause a true environmental problem with harmful and sometimes irreversible consequences on the watery ecosystem. The objective of this research is to evaluate the chemical pollution of the liquid effluents of the Hospital National Center and Academic Hubert KOUTOUKOU MAGA (CNHU-HKM) of Cotonou by the test of cytotoxicity based on the inhibition of the growth of the onions roots put in culture. The test of cytotoxicity carried out on the samples of hospital effluents of CNHU- HKM not cleansed showed that the Effective Concentration for which the chemical effects are observed for 50% of the individuals tested (EC50) is 22%. This result obtained reveals that the effluents of CNHU-HKM are chemically polluted, therefore is not in conformity with the standards as the vigor with the Benin one. These effluents were the subject of a depollution with three types of clays of Benin which are those of ETIGBO III, Gbedji-kotovi and MASSI. When clays are not purified, depollution is not effective. On the other hand when they are purified, depollution is effective especially in the case of the clay of ETIGBO III whose EC50 passed from 50% to 80%. This study shows the urgency and the interest of a control and a regular follow-up of these effluents at the exit of the station of purification of the hospital before being rejected into the urban networks of cleansing. Copyright@ International Science Community Association