International Science Community Association International Research Journal of Biological Sciences 2278-3202 9 3 2020 08 10 Satureja thymbra aqueous and ethanol extracts antibacterial activity 38 42 EN R Akaniro Ifunanya 1 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria H Anumudu Obinna 2 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria N Ofonegbu Mercy 3 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria P Nebo Aisha 4 Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria Mondal Astom 1 Department of Botany, University of Kalyani, Kalyani-741235, West Bengal, India Sinha Sankar Narayan 2 Department of Botany, University of Kalyani, Kalyani-741235, West Bengal, India Nnenna Omorodion 1 Department of Microbiology University of Port Harcourt P.M.B 5323 Port Harcourt Rivers State, Nigeria ijuo Philomena 2 Department of Microbiology University of Port Harcourt P.M.B 5323 Port Harcourt Rivers State, Nigeria P.V. Divya 1 Dept. in Microbiology, Manonmaniam Sundaranar University, Tirunelveli Rd, Abishekapatti, Tamil Nadu K. Sukesh 2 Dept. in Microbiology, Malankara Catholic College, Mariagiri, Tamil Nadu, India T. Ashitha 1 Research and Development Centre, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India K. Seedikkoya 2 M.E.S. Mampad College, Malappuram District-676 542, Kerala, India J.O. Arogbodo 1 Department of Animal Production and Health, Federal University of Technology, Akure, Ondo State, Nigeria I.A. Adebayo 2 Department of Animal Production and Health, Federal University of Technology, Akure, Ondo State, Nigeria J.A. Adesida 3 Department of Animal Production and Health, Federal University of Technology, Akure, Ondo State, Nigeria Abdallah Lubna 1 Department of Biology & Biotechnology, Faculty of Science, An-Najah National University, Nablus, P.O. Box 7, Palestine Omar Ghadeer 2 Department of Biology & Biotechnology, Faculty of Science, An-Najah National University, Nablus, P.O. Box 7, Palestine > Short Communication 2020 3 15 2020 08 10 The concern toward using herbs around the world for the treatment of infectious diseases is increased during this century. Based on that, the following conducted experiment was performed to detect the effectiveness of using ethanol and aqueous extracts from Satureja thymbra L. (Lamiaceae) that is growing wild in Palestine. The bioactivity of both aqueous and ethanol extracts was tested against Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and other two clinical isolates which are Klebsiella pneumoniae and Proteus mirabilis. Well susceptibility method and micro-broth dilution method were utilized to examine the antibacterial potential for both extract types under investigation. The obtained results showed that S. thymbra ethanol extract was better than aqueous one as it produced (10, 30, 16 and 12 mm) inhibition zones against K. pneumoniae, P. mirabilis, P. aeruginosa and S. aureus respectively. Meanwhile, S. thymbra aqueous extract had an impact on P. mirabilis with 20 mm zone of inhibition. The MIC results of the running experiment showed that ethanol extract exhibited a powerful inhibitory behavior as it prevented the growth of all tested microorganisms in a concentration range between 6.25 mg/ml and 25 mg/ml. Moreover, S. thymbra aqueous extract showed a moderate inhibition potential at 50 mg/ml MIC values to all examined bacterial isolates expect for K. pneumoniae clinical isolate. In conclusion, the acquired results confirmed the possibility of employing S. thymbra extracts in medicine and pharmaceutical industry of new drugs against some pathogenic bacteria. Copyright@ International Science Community Association