International Science Community Association International Research Journal of Biological Sciences 2278-3202 9 2 2020 05 10 Cytoprotective effects of the leaves aqueous extract of Combretum glutinosum (Combretaceae) on gastric ulcers in mice 6 11 EN Oludare Agbolade James 1 Department of Plant Science and Biotechnology, Federal University Oye Ekiti, Ekiti State, Nigeria Mary Popoola Kehinde 2 Department of Plant Science and Biotechnology, Federal University Oye Ekiti, Ekiti State, Nigeria Adebare Ganiyu Sikiru 3 Department of Agronomy, Federal University, Keshere, Gombe State, Nigeria Philip Olakunle Teju 4 Department of Applied Sciences, Osun State Polytechnic, Iree Rasheed Popoola Akinola 5 Department of Crop Protection, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria Sunday OJO Elijah 6 Department of Crop Protection, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria Ehis Imonmion Joshua 7 Department of Crop Protection, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria Perfusion Amang A. 1 Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon Luc Vandi V. 2 Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon Christophe Mezui 3 Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon Gael Siwe T. 4 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon Mesmine Kuissu T. 5 Institute of Medical Research and Study of Medicinal Plants, P.O. Box 13033 Yaoundé, Cameroon Vernyuy Tan P. 6 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon Perfusion Amang A. 1 Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon Luc Vandi V. 2 Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon Christophe Mezui 3 Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon Gael Siwe T. 4 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon Mesmine Kuissu T. 5 Institute of Medical Research and Study of Medicinal Plants, P.O. Box 13033 Yaoundé, Cameroon Vernyuy Tan P. 6 Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon > Research Paper 2019 9 18 2020 05 10 This work was designed to verify the cytoprotective property of the leaves aqueous extract of Combretum glutinosum (LAECG) on gastric ulcers (GU) in mice. The cytoprotective property of LAECG was evaluated by using three experimental methods of GU in mice: HCl/EtOH model, HCl/EtOH with indomethacin pretreatment model and indomethacin model. For each method, male mice were divided into 4 groups of 5 mice and treated respectively with distilled water (negative control), sucralfate (positive control) and extract (200 and 400 mg/kg). A qualitative phytochemical analysis was realized to detect some classes of compounds present in extract like alkaloids, phenolic compounds, flavonoids, terpenoids, tannins, and coumarins. C. glutinosum extract (400 mg/kg), showed his best activity in all the methods. Administration of LAECG (400 mg/kg) by oral route inhibited the formation of the GU of 27.84, 36.11 and 100%, respectively for the HCl/EtOH model, HCl/EtOH with indomethacin pretreatment model and indomethacin model. These percentages of inhibition of extract at 400 mg/kg were accompanied by the increase of mucus secretion of 97.50, 163.18 and 111.63%, respectively. Flavonoids, tannins and phenolic compounds were found to be present in LAECG. LAECG would protect the gastric mucosa membrane by stimulating mucus secretion through a mechanism which would not involve synthesis of endogenous prostaglandins, but rather by a direct effect on mucus secreting cells. Copyright@ International Science Community Association