International Science Community Association International Research Journal of Biological Sciences 2278-3202 9 1 2020 02 10 Ascertaining the genetic basis of variants in Justicia adhatoda L. collected from Tamil Nadu using ISSR- PCR Markers 17 20 EN Torres Hannah 1 Biology Department, Negros Oriental State University, Main Campus, Kagawasan Avenue, Dumaguete City, Negros Oriental, Philippines Kinamot-Bantoto Venus 2 Biology Department, Negros Oriental State University, Main Campus, Kagawasan Avenue, Dumaguete City, Negros Oriental, Philippines Subhranshu Shekhar 1 ITM University Gwalior, Madhya Pradesh, India Chitranshu Pandey 2 R&D, MRD Life Sciences Pvt. Ltd., Lucknow, Uttar Pradesh, India Pallavi Sharma 3 R&D, MRD Life Sciences Pvt. Ltd., Lucknow, Uttar Pradesh, India Shashikala Mishra 4 Department of Chemistry, Sacred Heart Degree College, Sitapur, Uttar Pradesh, India Jebarubi E. 1 St. Xavier′s College (Autonomous), Palayamkottai, Tamil Nadu, India Leon Stephan Raj T. 2 Plant Molecular Biology Research Unit, Department of Botany, St. Xavier′s College (Autonomous), Palayamkottai, Tamil Nadu, India > Short Communication 2019 4 1 2020 02 10 In the present investigation genetic variability of Justicia adhatoda L. belonging to the family Acanthaceae was carried out. The healthy fresh young leaves were collected from four different regions of Tamil Nadu. The DNA was isolated with standard protocol and the ISSR analysis was studied with five primers. The primers produced distinct bands in each population. The polymorphic loci number was 12 and percentage was 36 respectively. In between the population the genetic distance ranged from 0.3610 to 0.0625 and 0.9394 to 0.6970 respectively. 1.3636 was the mean value of overall observed number of alleles and 1.2788 was the effective number of alleles. The overall value for the genetic diversity was 0.1553 and value for Shannon information index was 0.2243. The UPGMA dendrogram represents the phylogenetic relationship between different accessions of Justicia adhatoda. The present study helps to find out the accession which has more genetic variability for the conservation in near future. Copyright@ International Science Community Association