@Research Paper <#LINE#>Forensic entomology and wildlife crime-the role of Blowfly larvae <#LINE#>Simon@T.M. <#LINE#>1-7<#LINE#>1.ISCA-IRJBS-2019-027.pdf<#LINE#>Department of Biological Sciences, University of Chester, UK<#LINE#>15/2/2019<#LINE#>1/7/2019<#LINE#>The importance of forensic entomology in crime scenes investigation cannot be underestimated. Through analysis of the morphology of blowfly larvae in comparison with the ambient environment at a death scene, critical information regarding a crime can be acquired. Interpretations can then be made in identifying the average time since the death of the particular animal- Post Mortem Interval. In conservation, therefore, the application of forensic techniques combined with other methods such as DNA analysis could help in identifying probable offenders thereby solving criminal acts involving animals and enhancing the protection of critically endangered species.<#LINE#>Guo Y.D., Cai J.F., Tang Z.C., Feng X.O., Lin Z., Yong F. and Wen J.F. (2011).@Application of Aldrichina grahami (Diptera, Calliphoridae) for forensic investigation in central-south China.@Romanian Journal of Legal Medicine, 19(1), 55-58. https://doi:10.4323/rjlm.2011.55@Yes$Pastula E.C. and Merritt R.W. (2013).@Insect Arrival Pattern and Succession on Buried Carrion in Michigan.@Journal of Medical Entomology, 50(2), 432-439. https://doi:10.1603/me12138@Yes$Rysavy N.M. and Goff M.L. (2015).@Preliminary Observations of Arthropods Associated with Buried Carrion on Oahu.@Journal of Forensic Sciences, 60(2), 462-467. https://doi:10.1111/1556-4029.12643@Yes$Anderson G.S. (2017).@Forensic entomology: The use of insects in death investigations.@Retrieved from https://www.sfu.ca/~ganderso/forensicentomology.htm. Accessed 2019-01-14@No$Anderson G.S. (2000).@Insect Succession on Carrion and its Relationship to Determining Time since Death.@In Forensic Entomology: The Utility of Arthropods in Legal Investigations. Castner, E. and Byrd, J. (Eds.) CRC Press. 143-176. https://doi.org/10.1201/9781420036947.ch5@Yes$Li L.L., Wang Y., Wang J.F., Ma M.Y. and Lai Y. (2016).@Temperature-dependent development and the significance for estimating postmortem interval of Chrysomya nigripes Aubertin, a new forensically important species in China.@International Journal of Legal Medicine, 130(5), 1363-1370. https://doi:10.1007/s00414-016-1315-6@Yes$Gamber M. (2006).@Bugs, Bodies, and Crime Scene Investigation.@Retrieved from http://tolweb.org/ treehouses/?treehouse_id=4197. Accessed 2019-02-10@No$Joseph I., Mathew D.G., Sathyan P. and Vargheese G. (2011).@The use of insects in forensic investigations: An overview on the scope of forensic entomology.@Journal of Forensic Dental Sciences, 3(2), 89-91. http://doi.org/10.4103/0975-1475.92154@Yes$El-Moaty Z.A. and Kheirallah A.M. (2013).@Developmental variation of the blow fly Lucilia sericata (Meigen, 1826) (Diptera: Calliphoridae) by different substrate tissue types.@Journal of Asia-Pacific Entomology, 16(3), 297-300. https://doi:10.1016/ j.aspen.2013.03.008@Yes$Picard C.J., Deblois K., Tovar F., Bradley J.L., Johnston J.S. and Tarone A.M. (2013).@Increasing Precision in Development-Based Postmortem Interval Estimates: What@Journal of Medical Entomology, 50(2), 425-431. https://doi:10.1603/me12051@Yes$SFU Museum of Archaeology and Ethnology (2010).@Investigating forensics - How are missing persons identified?.@Retrieved from http://www.sfu.museum/ forensics/eng/pg_media-media_pg/identification/. Accessed on 2019-02-14@No <#LINE#>Erythrogram, Leukogram and Blood cell measurement of Muscovy and Indian Runner Ducks - a comparison <#LINE#>C.P.@Acharya ,A.@Bhattacherjee ,B.K.@Mallik ,P.K.@Mohanty <#LINE#>8-16<#LINE#>2.ISCA-IRJBS-2019-032.pdf<#LINE#>P.G. Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar - 751 004 Odisha, India@P.G. Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar - 751 004 Odisha, India@Central Poultry Development Organisation, (ER), Bhubaneswar- 751 012 Odisha, India@P.G. Department of Zoology, Utkal University, Vani Vihar, Bhubaneswar - 751 004 Odisha, India<#LINE#>25/2/2019<#LINE#>16/7/2019<#LINE#>The present study discusses and tries to compare hematological and morphometrical parameters of blood cells between Muscovy (MY) and Indian Runner (IR) ducks. Sample of blood were collected from forty adult birds (ten drakes and ten ducks belonging to each species). Hb%, TEC, heterophil %, Nucleus Breadth of lymphocytes, Nucleus: Cytoplasm ratio of lymphocytes and Cell Length/Cell Breadth ratio of heterophils significantly differs at p<0.05. But, percentages of monocytes, eosinophils and basophils, morphometric parameters of i. RBC (except Cell Length/Cell Breadth), ii. lymphocytes (except Nucleus Breadth and Nucleus: Cytoplasm), iii. monocytes, iv. eosinophils, v. heterophils (except Cell Length/Cell Breadth) and vi. Cell Breadth of basophils are significantly different at p<0.01 between individuals of two species and males, females of each species. Other parameters, except those mentioned above do not differ significantly.<#LINE#>Livezey B.C. and Zushi R.L. (2007).@Higher order phylogeny of modern birds (Therapoda, Aves: Neornithes) based on comparative anatomy. II. Analysis and discussion.@Zool. J. Linnean. Soc., 149(1), 1-95. https://doi.org/10.1111/j.1096-3642.2006.00293.x.@Yes$Howard L. Anatidae (On-line) (2019).@Animal Diversity.@Web http://animaldiversity.org/accounts/Anatidae/. 23/02/2019.@No$Langlois, C. (2004).@Marvelous Muscovies.@Mother Earth News, http://www.motherearthnews.com/homesteading-and-livestock/marvelous muscovies-zmaz04onzsel.aspx. 23/02/2019.@Yes$Coates W.S., County S. and Ernst R.A. (2000).@Raising Ducks in Small Flocks.@Division of Agriculture and Natural Resources, Cooperative Extension, University of California, Publication 2980. 1-10.@No$Clark Phillip, Boardman Wayne and Raidal Shane (2009).@Atlas of Clinical Avian Hematology.@Wiley- Blackwell, West Sussex, United Kingdom, 1, 19-22, 71-74. ISBN: 978-1-4051-9248-4@Yes$Hartman F.A. and Lessler M.A. (1963).@Erythrocyte measurement in birds.@Auk., 80(4), 467-473. https://doi.org/10.2307/4082852.@Yes$Atatür M.K., Arikan H. and Çevik I.E. (1999).@Erythrocyte sizes of some anurans from Turkey.@Turk. J. Zool., 23(2), 111-114. URL: journals.tubitak.gov.tr/zoology/ abstract.htm?id=3074.@Yes$Nowaczewski S. and Kontecka H. (2012).@Haematological indices, size of erythrocytes and haemoglobin saturation in broiler chickens kept in commercial conditions.@Anim. Sci. Pap. Rep., 30(2), 181-190. URL: agro.icm.edu.pl/agro/ element/bwmeta1.element.agro-0c261089-6e3c-45c5-91a4-83f459fe9afc.@Yes$Narkkong N.A., Aengwanich W. and Tanomthong A. (2011).@Morphology and morphometrics of hematological cells from eastern sarus crane.@Grusantigonesharpii. Comp. Clin. Path., 20(4), 299-304. https://doi.org/10.1007/ s00580-010-0990-9.@Yes$Sonia C., Rajini R.A., Babu M. and Vairamuthu S. (2012).@The effect of age, sex and rearing system on differential count in Guinea fowl.@Indian. J. Poult. Sci., 47(2), 251-253. URL: www.indianjournals.com/ Mobile/SearchResult.aspx?query=1@Yes$Campbell Terry W. (1994).@Hematology. 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URL: www.sokvetjournal.net/index.php/ past-issues?id=214.@Yes$Lazăr R., Boişteanu P.C., Muntean C., Apetroaei (Petrescu) C-A. and Ciobanu M.M. (2012).@Characterisation of the haematological profile of the hybrid B.U.T 6 turkey raised in Romania.@Lucrări Ştiinţifice - Seria Zootehnie, 58(17), 254-257. URL: www.uaiasi.ro/zootehnie/en/journal/vol-58/Roxana_Lazar.html.@Yes$Nalubamba K.S., Mudenda N.B. and Masuku M. (2010).@Indices of health; clinical haematology and body weights of free-range Guinea fowl (Numida meleagris) from the southern province of Zambia.@Int. J. Poult. Sci., 9(12), 1083-1086. 10.3923/ijps.2010.1083.1086.@Yes$Obinna O.V.N., Emmanuel O.U., Princewill O.I., Helen O. and Christopher E. (2011).@Effect of sex and systems of production on the hematological and serum biochemical characters of helmeted guinea fowls (Numida meleagris pallas) in South Eastern Nigeria.@Int. J. Biosci., 1(3), 51-56. 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(2015).@Comparative haematology of Anasplatyrhynchos (Anseriformes) and Coturnixcoturnix japonica (Galliformes).@J. Entomol. Zool. Stud., 3(5), 50-53. http://dx.doi.org/10.22271/j.ento.@Yes$Utama I.H., Sugiyarto Kendran A.A.S., Apsari I.A.P., Suarsana I.N., Erawan I.G.M.K., Adi A.A.A.M., Winaya I.B.O. and Hayashi Y. (2008).@Blood smear evaluation of Bali ducks sampled from traditional farming systems in Bali.@J. Vet., 9(4), 188-191. URL: https://ojs.unud.ac.id/ index.php/jvet/article/view/3334.@Yes$Coke R.L., West G.D. and Hoover J.P. (2004).@Hematology and plasma biochemistry of captive Puna Ibis (Plegadisridgewayi).@J. Wildl. Dis., 40(1), 141-144. https://doi.org/10.7589/0090-3558-40.1.141.@Yes$Travis E.K., Vargas F.H., Merkel J., Gottdenker N., Miller R.E. and Parker P.G. (2006).@Hematology, serum chemistry and serology of Galapagos penguins (Spheniscusmendiculus) in the Galapagos Islands, Ecuador.@J. Wildl. Dis., 42(3), 625-632. https://doi.org/10.7589/0090-3558-42.3.625.@Yes$Charles-Smith L.E., Rutledge M.E., Meek C.J., Baine K., Massey E., Ellsaesser L.N., DePerno C.S., Moorman C.E. and Degernes L.A. (2014).@Hematologic parameters and hemoparasites of nonmigratory Canada geese (Brantacanadensis) from Greensboro, North Carolina, USA.@J. Avian. Med. Surg., 28(1), 16-23. https://doi.org/10.1647/2012-072.@Yes$Tadjalli M., Nazifi S. and Eemanparvar A. (2003).@Normal cellular morphology of the blood of Japanese quail (Coturnixcoturnix japonica).@CompClin Path., 12(2), 102-105. https://doi.org/10.1007/s00580-003-0485-z.@Yes$Mushi E.Z., Binta M.G., Chabo R.G., Isa J.F.W. and Kappata R.W. (1999).@Selected haematological values of farmed ostriches (Struthiocamelus) in Bostwana.@J. Vet. Diagn. Invest., 11(4), 372-374. https://doi.org/10.1177/ 104063879901100415@Yes$Tadjalli M., Nazifi S., Abbasabadi B.M. and Majidi B. (2013).@Histomorphometric study on blood cells in male adult ostrich.@Vet. Res. Forum., 4(3), 199-203. URL: vrf.iranjournals.ir/article_2819.html.@Yes$Sabino A.J., Trevelin S.C., Almeida B.F.M., Peiró J.R. and Ciarlini P.C. (2011).@Erythrogram and erythrocytes measurement of ostriches (Struthiocamelus) in São José do Rio Preto-SP, Brazil.@Braz. J. Vet. Res. Anim. Sci. São Paulo., 48(3), 234-238. https://doi.org/10.11606/S1413-95962011000300008.@Yes$Keçeci* T. and Çöl R. (2011).@Haematological and biochemical values of the blood of pheasants (Phasianuscolchicus) of different ages.@Turk J Vet Anim Sci., 35(3), 149-156. URL: journals.tubitak.gov.tr/ veterinary/abstract.htm?id=11673.@Yes$Polo F.J., Celdran J.F., Peinado V.I., Viscor G. and Palomeque J. (1992).@Hematological values for four species of birds of prey.@Condor., 94(4), 1007-1013. 10.2307/1369300.@Yes$Tok C.V., Tosunoğlu M., Ayaz D., Çiçek K. and Gül Ç. (2009).@Hematology of Lycian Salamander, Lyciasalamandra fazilae.@North. West. J. Zool., 5(2), 321-329. URL: biozoojournals.ro/nwjz/content/v5.2/nwjz. 051127.Tok.pdf.@Yes$Arikan H. and Çiçek K. (2010).@Morphology of peripheral blood cells from various species of Turkish herpetofauna.@Acta.Herpetol., 5(2), 179-198. http://dx.doi.org/10.13128/ Acta_Herpetol-9032.@Yes$Orós J., Casal A.B. and Arencibia A. (2010).@Microscopic studies on characterization of blood cells of endangered sea turtles.@In Microscopy: Science, Technology, Applications and Education (eds. Méndez-Vilas A. and Díaz J.), Formatex Research Center, Badajoz, Spain, 75-84. ISBN:13: 978-84-614-6191-2@Yes$Radhakrishnan S., Stephen M. and Nair N.B. (1976).@A study on the blood cells of a marine teleost fish, Diodon Hystrixtogether with a suggestion as to the origin of lymphocytes.@Proc. Indian. Natl. Sci. Acad., 42 (4and5), 212-226. URL: https://insa.nic.in/writeraddata/ UpLoadedFiles/PINSA/Vol42B_1976_4and5_Art08.pdf@Yes$Ponsen S., Narkkong N.A., Pamok S. and Aengwanich W. (2009).@Comparative Hematological Values, Morphometric and Morphological Observation of the Blood Cell in Capture and Culture Asian Eel, Monopterusalbus (Zuiew).@Am. J. Anim. Vet. Sci., 4(2), 32-36. 10.3844/ajavsp.2009.32.36@Yes$Homątowska A., Wojtaszek J. and Adamowicz A. (2002).@Haematological indices and circulating blood picture in the Sunbleak, Leucaspiusdelineatus (Heckel, 1843).@Zool. Pol., 47(3-4), 57-68. http://polona.pl/item/45884678.@Yes <#LINE#>Treatment of dye house effluents by a developed bacterial consortium: A shake flask study <#LINE#>Patel@Darshna K. ,Tipre@Devayani R. ,Dave@Shailesh R. <#LINE#>17-25<#LINE#>3.ISCA-IRJBS-2019-033.pdf<#LINE#>Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, India@Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad 380009, Gujarat, India@Xavier's Research Foundation, Loyola Centre for Research and Dev., St. Xavier College Campus, Navrangpura, Ahmedabad, Gujarat, India<#LINE#>13/3/2019<#LINE#>18/8/2019<#LINE#>Bacterial consortium developed for decolourising 18 different metal complex dyes was used to investigate its efficiency towards treatment of three different dye house effluents E-1, E-4 and E-5(DHEs). Process was optimised at shake flask level for the pH, temperature, culture condition, carbon and nitrogen source. The developed consortium showed better decolourisation of the DHEs under static condition compared to shaking condition between pH 6-10 and 25-45C temperature. Decolourization was positively influenced by the addition of glucose, sucrose, maltose and starch in case of E-1, whereas E-5 was found to be better decolourized by supplementation of beef extract to the basal medium. However, E-4 showed variable decolourization pattern with <60% decolourization irrespective of carbon and nitrogen sources used. Decolourization and degradation profiles of all the 3DHEs were studied to optimize treatment time. Reduction in BOD, COD and American Dye Manufacturers\' Institute (ADMI) values were in the range of 60-85% except for E-4 where BOD and ADMI removal was in the range of 75-90%, but rest of the other parameters were reduced in the range of 20-60% which were lost. Moreover, FTIR and HPLC spectral data analysis and enzyme induction pattern confirmed biodegradation of all the DHEs. Considerable amount of intracellular azoreductase, NADH-DCIP reductase and laccase productions were detected, which played significant role in degradation. Reduction in phyto- and microbial toxicity was found in the range of 70-100% and 40-53%, respectively which indicated detoxification of the wastes. The findings indicate that the developed bacterial consortium can be used for bio tretament of recalcitrant DHEs.<#LINE#>Nigam P., Banat I.M., Singh D. and Marchant R. (1996).@Microbial process for the decolorization of textile effluent containing azo, diazo and reactive dyes.@Process Biochem., 31, 435-442.@Yes$Tehrani-Bagha A., Mahmoodi N. and Menger F. (2010).@Degradation of a persistent organic dye from colored textile wastewater by ozonation.@Desalination, 260, 34-38.@Yes$Li H., Liu F., Zhu M., Feng X., Zhang J. and Yin H. (2015).@Structure and properties of Co-doped cryptomelane and its enhanced removal of Pb2+ and Cr3+ from wastewater.@J. Environ. Sci., 34, 77-85.@Yes$Rai H., Bhattacharya M., Singh J., Bansal T.K., Vats P. and Banerjee U.C. (2005).@Removal of dyes from the effluent of textile and dyestuff manufacturing industry: A review of emerging techniques with reference to biological treatment.@Crit. Rev. Environ. Sci. Technol., 35, 219-238.@Yes$Verma P. and Madamwar D. (2003).@Decolorization of synthetic dyes by a newly isolated strain of Serratia maerascens.@World J. Microbiol. Biotechnol., 19, 615-618.@Yes$Dave S.R. and Dave R.H. (2009).@Isolation and characterization of Bacillus thuringiensis for Acid red 119 dye decolourisation.@Bioresour. Technol., 100(1), 249-253.@Yes$Yang Q., Li C., Li H., Li Y. and Yu N. (2009).@Degradation of synthetic reactive azo dyes and treatment of textile wastewater by a fungi consortium reactor.@Biochem. Eng. J., 43, 225-230.@Yes$Zablocka-Godlewska E., Przystas W. and Grabinska-Sota E. (2014).@Dye decolourisation using two Klebsiella strains.@Water, Air, Soil Pollut., 226, 2249.@Yes$de Almeida E.J.R. and Corso C.R. (2016).@Acid Blue 161: Decolorization and toxicity analysis after microbiological treatment.@Water, Air, Soil Pollut., 227, 468-476.@Yes$Alizadeh N., Shariati S. and Besharati N. (2017).@Adsorption of crystal violet and methylene blue on Azolla and Fig leaves modified with magnetite iron oxide nanoparticles.@Int. J. Environ. Res., 11(2), 197-206.@Yes$Ghosh A., Dastidar M.G. and Sreekrishnan T.R. (2017).@Bioremediation of chromium complex dyes and treatment of sludge generated during the process.@Int. Biodeter. Biodegr., 119, 448-460.@Yes$Khehra M., Saini H., Sharma D., Chadha B. and Chimni S. (2005).@Decolorization of various azo dyes by bacterial consortium.@Dyes Pigments., 67, 55-61.@Yes$Sheth N.T. and Dave S.R. (2010).@Enhanced biodegradation of Reactive Violet 5R manufacturing wastewater using down flow fixed film bioreactor.@Bioresour. Technol., 101, 8627-8631.@Yes$Balapure K., Jain K., Bhatt N. and Madamwar D. (2016).@Exploring bioremediation strategies to enhance the mineralization of textile industrial wastewater through sequential anaerobic-microaerophilic process.@Int. Biodeter. Biodegr., 106, 97-105.@Yes$Patel D.K., Tipre D.R. and Dave S.R. (2017).@Selection and development of efficient consortia for decolorization of metal complex dyes.@Toxicol. Environ. Chem., 99(2), 252-264.@Yes$Patel D.K., Tipre D.R. and Dave S.R. (2017).@Enzyme mediated bacterial biotransformation and reduction in toxicity of 1:2 chromium complex AB193 and AB194 dyes.@J. Taiwan Inst. Chem. Eng., 77, 1-9.@Yes$Patel D.K., Tipre D.R., Patel T.L., Kadam A., Agarwal S.A. and Dave S.R. (2018).@Elucidation of biochemical mechanism involved in microbial degradation of 1:1 metal complex dye containing simulated wastewater.@Int. J. Curr. Microbiol. App. Sci., 7(3), 2774-2789.@No$Eaton A.D., Clesceri L.S., Greenberg A.E. and Franson M.A.H. (1998).@Standard methods for the examination of water and wastewater.@19th ed. Washington, DC: American Public Health Association.@No$Khan Z., Jain K., Soni A. and Madamwar D. (2014).@Microaerophilic degradation of sulphonated azo dye Reactive Red 195 by bacterial consortium AR1 through co-metabolism.@Int. Biodeter. Biodegr., 94, 167-175.@Yes$Lade H.S., Waghmode T.R., Kadam A.A. and Govindwar S.P. (2012).@Enhanced biodegradation and detoxification of disperse azo dye Rubine GFL and textile industry effluent by defined fungal-bacterial consortium.@Int. Biodeter.Biodegr., 72, 94-107.@Yes$Shah P.D., Dave S.R. and Rao M.S. (2012).@Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain Alcaligenes faecalis PMS-1.@Int. Biodeter. Biodegr., 69, 41-50.@Yes$Agrawal S., Tipre D., Patel B. and Dave S. (2014).@Optimization of triazo Acid Black 210 dye degradation by Providencia sp. SRS82 and elucidation of degradation pathway.@Process biochem., 49(1), 110-119.@Yes$Kadam A.A., Telke A.A., Jagtap S.S. and Govindwar S.P. (2011).@Decolorization of adsorbed textile dyes by developed consortium of Pseudomonas sp. SUK1 and Aspergillus ochraceus NCIM-1146 under solid state fermentation.@J. Hazard. Mater., 189(1), 486-494.@Yes$Patel T.L., Patel B.C., Kadam A.A., Tipre D.R. and Dave S.R. (2015).@Application of novel consortium TSR for treatment of industrial dye manufacturing effluent with concurrent removal of ADMI, COD, heavy metals and toxicity.@Water Sci. Technol., 71(9), 1293-1300.@Yes$Dhall P., Kumar R. and Kumar A. (2012).@Biodegradation of sewage wastewater using autochthonous bacteria.@Sci. World J., 1-8.@Yes$Pandey A., Singh P. and Iyengar L. (2007).@Bacterial decolorization and degradation of azo dyes.@Int. Biodeter. Biodegr., 59(2), 73-84.@Yes$Kurade M.B., Waghmode T.T., Kagalkar A.N. and Govindwar S.P. (2012).@Decolorization of textile industry effluent containing disperse dye Scarlet RR by a newly developed bacterial-yeast consortium BL-GG.@Chem. Eng. J., 184, 33-41.@Yes$Saratale R.G., Gandhi S.S., Purankar M.V., Kurade M.B., Govindwar S.P., Oh S.E. and Saratale G.D. (2012).@Decolorization and detoxification of sulfonated azo dye C.I. Remazol Red and textile effluent by isolated Lysinibacillus sp. RGS.@J. Biosci. Bioeng., 115(6), 658-667.@Yes$Khandare R.V., Kabra A.N., Kadam A.A. and Govindwar S.P. (2013).@Treatment of dye containing wastewaters by a developed lab scale phytoreactor and enhancement of its efficacy by bacterial augmentation.@Int. Biodeter. Biodegr., 78, 89-97.@Yes$Zhang W., Liu W., Zhang J., Zhao H., Zhang Y., Quan X. and Jin Y. (2012).@Characterisation of acute toxicity, genotoxicity and oxidative stress posed by textile effluent on zebra fish.@J. Environ. Sci., 24(11), 2019-2027.@Yes$Hassan M.M. and Hawkyard C.J. (2002).@Ferral-catalyzed ozonation of aqueous dyes in a bubble-column reactor.@Catalysis Communications, 3(7), 281-286.@Yes$Singh R.L., Singh P.K. and Singh R.P. (2015).@Enzymatic decolorization and degradation of azo dyes - A review.@Int. Biodeter. Biodegr., 104, 21-31.@Yes <#LINE#>A novel, low cost, sterilizable and disposable in-house culture vessel for laboratory study of microalgae cultures <#LINE#>Paul@Vishal ,Dwivedi@Vinay ,Saxena@Nishant ,Kodgire@Santosh ,Chudasama@Yogesh ,Chawada@Hardik ,Desai@, Dishant ,Shanmugasundaram@Suguna ,Shanmugasundaram@Sambandham ,Sanyal@Debanjan ,Dasgupta@Santanu <#LINE#>26-31<#LINE#>4.ISCA-IRJBS-2019-034.pdf<#LINE#>Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Industries Ltd, Jamnagar-361140, India@Research and Development, Reliance Corporate Park, Ghansoli, Mumbai-400709, India<#LINE#>22/3/2019<#LINE#>28/7/2019<#LINE#>Algal industry is expanding as a source of energy, food, feed, recombinant proteins and high value chemicals. As the research need is increasing, many laboratories are getting involved. Photobioreactors are used to achieve uniform light illumination and CO2 supply to achieve faster algal growth. Use of commercially available PBR in algal study is limited to few highly funded laboratories due to large initial capital requirement and high operational cost. To address the issue of uniform light illumination and CO2 supply at low cost, an In-house Culture Vessel (ICV) has been designed and the algal growth was compared between ICV vs. conventional 20L carboys which are generally used in lab for relatively larger quantity of inoculum development. An in-built air-sparger has been provided to achieve both air lift mixing of culture and CO2 supply. The well mixed culture with high surface area allows uniform light illumination. Picochlorum sp. was grown in the designed culture vessel and in conventional carboys. Growth rate in ICV was 16 times more as compared to carboy. This enhanced growth rate indicates more and/or even availability of light to algal cells in ICV compared to carboy as the starting nutrient level was same in both. Growth rate of 0.16 OD/day even at 35 OD in ICV indicates that the culture of Picochlorum sp is still in growing phase in ICV whereas the same culture reached stationary phase in the 20L carboy at 3.73 OD with negligible growth rate of 0.02 OD/day. The proven fact of chlorophyll a/b ratio adjustment in response to light was observed as well with maintained Nitrogen level for Picochlorum sp. The In-House Culture Vessel was demonstrated to be a promising and inexpensive tool which could be used as a closed system for developing actively growing high density microalgae cultures at laboratory scale for further studies.<#LINE#>Koller M., Muhr A. and Braunegg G. (2014).@Microalgae as versatile cellular factories for valued products.@Algal Res., 6 (Part A), 52-63.@Yes$Lauersen K.J., Huber I., Wichmann J., Baier T., Leiter A., Gaukel V., Kartushin V., Rattenholl A., Steinweg C., Von Riesen L., Posten C., Gudermann F., Lütkemeyer D., Mussgnug J.H. and Kruse O. (2015).@Investigating the dynamics of recombinant protein secretion from a microalgal host.@J. Biotechnol, 215, 62-71. doi: 10.1016/j.jbiotec.2015.05.001.@Yes$Posten C. (2009).@Design principles of photo-bioreactors for cultivation of microalgae.@Eng. Life Sci., 9, 165-177.@Yes$Vasumathi K.K., Premalatha M. and Subramanian P. (2012).@Parameters influencing the design of photobioreactor for the growth of microalgae.@Renew. Sust. Energ. Rev., 16(7), 5443-5450.@Yes$Mooij De T., de Vries G., Latsos C., Wijffels R.H. and Janssen M. (2016).@Impact of light color on photobioreactor productivity.@Algal Res, 15, 32-42.@Yes$Baer S., Heining M., Schwerna P., Buchholz R. and Hübner H. (2016).@Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor.@Algal Res., 14, 109-115.@Yes$Takache H., Pruvost J. and Marec H. (2015).@Investigation of light/dark cycles effects on the photosynthetic growth of Chlamydomonas reinhardtii in conditions representative of photobioreactor cultivation.@Algal Res., 8, 192-204.@Yes$Pegallapati A.K., Arudchelvam Y. and Nirmalakhandan N. (2012).@Energy-efficient photobioreactor configuration for algal biomass production.@Bioresour. Technol, 126, 266-273.@Yes$Ogbonna J.C. and Tanaka H. (2000).@Light requirement and photosynthetic cell cultivation - Development of processes for efficient light utilization in photobioreactors.@J. Appl. Phycol., 12, 207-218.@Yes$Wang B., Lan C.Q. and Horsman M. (2012).@Closed photobioreactors for production of microalgal biomasses.@Biotechnol. Adv., 30, 904-912.@Yes$Singh R.N. and Sharma S. (2012).@Development of suitable photobioreactor for algae production - A review.@Renewable and Sustainable Energy Reviews, 16(4), 2347-2353.@Yes$Tredici M.R. (2007).@Mass Production of Microalgae: Photobioreactors.@In: Richmond A (ed), Handbook of Microalgal Culture: Biotechnology and Applied Phycology. Blackwell Publishing Ltd edn., Oxford, UK, 178-214.@Yes$Masuda T., Tanaka A. and Melis A. (2003).@Chlorophyll antenna size adjustments by irradiance in Dunaliella salina involve coordinate regulation of chlorophyll a oxygenase (CAO) and Lhcb gene expression.@Plant Molecular Biology, 51(5), 757-771. DOI: 10.1023/A:1022545118212.@Yes$Demmig‐Adams B. and Adams Iii W. (1992).@Photoprotection and other responses of plants to high light stress.@Annual review of plant biology, 43(1), 599‐626.@Yes$Hikosaka K. and Terashima I. (1995).@A model of the acclimation of photosynthesis in the leaves of C3 plants to sun and shade with respect to nitrogen use.@Plant, Cell and Environment, 18, 605-618.@Yes$Terashima I. and Evans J.R. (1988).@Effects of nitrogen nutrition on electron transport components and photosynthesis in spinach.@Australian Journal of Plant Physiology, 14, 59-68.@Yes$Dale M.P. and Causton D.R. (1992).@Use of the chlorophyll a/b ratio as a bioassay for the light environment of a plant.@Functional Ecology, 6, 190-196.@Yes$Kitajima K. and Hogan K.P. (2003).@Increases of chlorophyll a/b ratios during acclimation of tropical woody seedlings to nitrogen limitation and high light.@Plant, Cell & Environment, 26(6), 857-865. DOI: 10.1046/j.1365-3040.01017.x@Yes$Sukumaran P., Nulit R., Halimoon N., Simoh S., Omar H. and Ismail A. (2018).@Formulation of Cost-effective Medium Using Urea as a Nitrogen Source for Arthrospira platensis Cultivation under Real Environment.@Annual Research & Review in Biology, 22(2), 1-12. Article no.ARRB.38182; ISSN: 2347-565X, NLM ID: 101632869@Yes$Guillard RRL and Ryther JH (1962).@Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran.@Can. J. Microbiol., 8, 229-239.@Yes$Guillard R.R. (1975).@Culture of phytoplankton for feeding marine invertebrates.@In Culture of marine invertebrate animals, Springer, Boston, MA, 29-60.@Yes$Lee S.H., Chi Y.A., Beom H.J., Sang A.L., Ji-Yeon P., Kwang G.A. and Hee-Mock O. (2013).@Increased Microalgae Growth and Nutrient Removal Using Balanced N:P Ratio in Wastewater.@J. Microbiol. Biotechnol, 23(1), 92-98. ISSN 1017-7825 eISSN 1738-8872@Yes$Choi H.J. and Lee S.M. (2014).@Effect of the N/P ratio on biomass productivity and nutrient removal from municipal wastewater.@Bioprocess Biosyst Eng, 38(4), 761-766. ISSN 1615-7591; DOI 10.1007/s00449-014-1317-z@Yes$Environmental Protection Agency (EPA) (1994).@United States. "Shortterm methods for measuring the chronic toxicity of effluents and receiving waters to freshwater organisms.@3&supa; ed. Cincinati, OH. U.S. Environmental Protection Agency, 1994. EPA 600/4-91/002.@No$Eaton A.D., Clesceri L.S. and Greenberg A.E. (1995).@Standard Methods for the Examination of Water and Wastewate.@ed. American Public Health Association, Washington, 19.@No$Šesták Z. (1971).@Determination of chlorophylls a and b. In Plant Photosynthetic Production.@Manual of Methods. Dr. W. Junk Publishers, The Hague, (Z. Šesták, J. Catsky, and P.G. Jarvis, eds.), 672-701.@No$Lichtenthaler H.K. (1987).@Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes.@Methods Enzymol, 148, 350-382.@Yes$Dagley S. and Hinshelwood C.N. (1938).@Physico-chemical aspects of bacterial growth.@Part I. Dependence of growth of Bact. kcctis aerogenes on concentration of medium, J. chem. Soc., 1930.@Yes$Monod J. (1942).@Recherches sur la croissance des cultures bacmnes.@Paris: Hermann et Cie.@Yes$Powell E.O. (1956).@Growth rate and generation time of bacteria, with special reference to continuous culture.@Microbiology, 15(3), 492-511.@Yes <#LINE#>Abiotic factors influencing the diversity and abundance of protozoans at two different stations of Devika stream in Udhampur district, J&K, India <#LINE#>Gupta@Shiwali ,kour @Sarbjeet ,Devi@Sushma <#LINE#>32-38<#LINE#>5.ISCA-IRJBS-2019-037.pdf<#LINE#>Department of Zoology, University of Jammu, Jammu-180006, India@Department of Zoology, University of Jammu, Jammu-180006, India@Department of Zoology, University of Jammu, Jammu-180006, India<#LINE#>25/3/2019<#LINE#>15/7/2019<#LINE#>Two different study sites of Devika stream were analysed for a period of one year (Oct, 2014 to Sept, 2015), in order to estimate the effect of various changing physico-chemical parameters on the diversity and abundance of inhabitant protozoans. During the present investigation, qualitatively, a total of 10 genera and 13 species viz. Centropyxis aculeata, C. ecornis, Paramecium aurelia, P. trichium, Vorticella sps., Epistylis plicatilis, Campanella umbellaria, Euplotes sps., Bursaridium schewakofii, Difflugia lebes, D. acuminata, Arcella vulgaris, Colpidium sps. were enlisted; out of which, only 6 genera showed their presence at Station II. Quantitative estimation revealed higher protozoan count at Station I (i.e:- 68.96%) than at station II. This may be attributed to the presence of comparatively higher levels of water temperature, free carbon-dioxide, sulphates, phosphates, nitrates, BOD as well as lower levels of DO at Station I. Furthermore, presence of some of the species like Epistylis plicatilis, Euplotes sps., Bursaridium schewakofii, Difflugia lebes and Arcella vulgaris at Station I infers to more anthropogenic influence at this station and also due to direct organic input. This organic load gets further diluted while travelling towards station II and seems less detrimental from the present data.<#LINE#>Murugan N., Murugavel P. and Kodarkar M.S. (1998).@Cladocera: The biology, classification, identification and ecology.@Indian Association of Aquatic Biologists (IAAB), Hyderabad, 1-47.@Yes$Rocha O., Matsumura-Tundisi T., Espindola E.L.G., Roche K.F. and Rietzler A.C. (1999).@Ecological theory applied to reswervoir zooplankton.@In: Theoretical reservoir ecology and its application (Eds.: J.G. Tundisi and M.Straskraba). Internat, Inst. Ecol., Sao Carlos, 457-476.@Yes$Dadhick N. and Saxena M.M. (1999).@Zooplankton as indicators of trophical status of some desert water near Bikanar.@J. Environ. Pollut., 6, 251-254.@Yes$Pawar D.H. (2012).@Physico-chemical status of the water of historical lakes and tanks in Kolhapur city.@Review of Research, 1, 1-4.@Yes$A.P.H.A. (1985).@Standard methods for the examination of water and waste water.@American Public Health Association, American Water Works Association and Water Pollution Control Federation, Washington, D.C., 1198.@No$Edmondson W.T. and Weinberg G.G. (1971).@A manual on productivity in Freshwaters.@Blackwell Scientific Publications, Oxford, 358.@No$Pennak R.W. (1978).@Fresh water invertebrates of United States.@2nd edition. A Wiley- Interscience Publication.@Yes$Adoni A.D. (1985).@Workbook on Limnology.@Pratibha Publishers, C-10 Gour Nagar Sagar, India.@Yes$Shukla U. and Gupta P.K. (2001).@Assemblage of ciliated protozoan community in a polluted and non-polluted environment in a tropical lake of central Himalaya: Lake Naini Tal, India.@Journal of Plankton Research, 23(6), 571-584.@Yes$Salvado H. and Gracia M.P. (1991).@Response of ciliate population to changing environmental conditions along a fresh water reservoir.@Arch. Hydrobiol., 123, 239-255.@Yes$Gupta S., Kour S. and Kaur H. (2015).@Seasonality of invertebrate fauna inhabiting lower Shiwalik stream, Philodinavus paradoxus- the first report.@Golden Research Thoughts, 4(7), 1-8.@No$Chander M.V. (2017).@Status of animal resources in relation to Hydro-biological variables in Chenani hydroelectric reservoir and its feeding channel.@Ph. D Thesis, University of Jammu, Jammu.@Yes <#LINE#>GC- MS analysis of secondary metabolites from acetone and chloroform extract of Dicranopteris linearis (burm. F.) Underw. <#LINE#>Vijayakumari @J. ,Raj@T. Leon Stephan <#LINE#>39-43<#LINE#>6.ISCA-IRJBS-2019-055.pdf<#LINE#>Plant Molecular Biology Research Unit, Department of Botany, St. Xavier's College (Autonomous), Palayamkottai- 627002, Affiliated to Manonmaniam Sundaranar University, Abhisekapatti Tirunelveli, Tamil Nadu, India@Plant Molecular Biology Research Unit, Department of Botany, St. Xavier's College (Autonomous), Palayamkottai- 627002, Affiliated to Manonmaniam Sundaranar University, Abhisekapatti Tirunelveli, Tamil Nadu, India<#LINE#>13/5/2019<#LINE#>6/8/2019<#LINE#>In the present study, the possible bioactive compounds recognized by gas chromatography and mass spectrometry (GC-MS) analysis in aerial parts of acetone and chloroform extract of Dicranopteris linearis (burm.f.) Under was investigated. The acetone and chloroform extract of D. linearis were explored thirteen bioactive compounds. The prevailing compounds in acetone extract were 3-Ethoxyacrylonitrile (61.93%), Indole-2-one, 2, 3-dihydro-N-hydroxy-4-methoxy-3, 3-dimethyl- (10.78%), Silicic acid, diethyl bis (trimethylsilyl) ester (14.43%) and 2-Ethylacridine (12.85%). The prevailing compounds in chloroform extract were 1H-Inden-1-ol, 2,3-dihydro- (2.88%), Phenol, 2,5-bis(1,1-dimethylethyl)- (6.48%), Heptacosane (2.20%), Di-n-decylsulfone (6.43%), Ethanone, 2-(2-benzothiazolylthio)-1-(3,5-dimethylpyrazolyl)- (15.24%), 1-Bromoeicosane (15.11%), Methoxyacetic acid, 2-tridecyl ester (19.05%), Octadecane, 3-ethyl-5-(2-ethylbutyl)- (20.59%) and 1,2,4-Benzene tricarboxylic acid, 4-dodecyl dimethyl ester (12.02%). The outcome of the present work may be useful in metabolomics research, nutraceuticals, and phytopharmaceuticals to evaluate their quality.<#LINE#>Singh L., Somarjit S., Singh K.S. and Jadu E. (2001).@Ethnomedicinal uses of some pteridophytic species in Maipur.@Indian Fern J., 18(1), 14-17.@Yes$Khare C.P. (2007).@Indian medicinal plants.@Springer science. New Delhi. 145.@Yes$Bilia A.R., Bergonzi M.C., Lazari D. and Vincieri F.F. (2002).@Characterization of commercial kava-kava herbal drug and herbal drug preparations by means of nuclear magnetic resonance spectroscopy.@J Agric Food Chem., 50, 5016-5025.@Yes$Manickam V.S. and Irudayaraj V. (1992).@Pteridophyte Flora of Western Ghats-South India.@B.I Publications Pvt Ltd.@Yes$Manickam V.S. and Irudayaraj V. (2003).@Pieridophytic flora of Ni/gins South India.@Bishen Singh Mahendra Pal Singh, 192.@Yes$Kumar P.P., Kumaravel S. and Lalitha C. (2010).@Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo.@Afr J Biochem Res., 4(7), 191-195.@Yes$Yasar A., Ucencu O., Gulec C., Inceer H., Ayaz S. and Yayal N. (2005).@GC-MS analysis of chloroform extracts in flowers, stems and roots of Tripleurospermum callosum.@Pharma Biol., 43(2), 108-112.@Yes$Prabhu T.P., Panneerselvam P., Suresh R., Atlee W.C. and Balasubramanian S. (2013).@GC-MS analysis of ethanolic extract of Canthium parviflorum Lamk Leaf.@Journal of Applied Pharmaceutical Science, 3 (2), 166-168.@Yes$Fiehn O., Kopka J., Dormann P., Altmann T., Trethewey R.N. and Willmitzer L. (2000).@Metabolic profiling for plant functional genomics.@Nat Biotechnol., 18, 1157-1161.@Yes$Liu T.B., Pan M.Y., Ling X.F., Zhang Y., Zhang C. and Bi S.F. (2013).@GC-MS analysis of chemical constituents of volatile oil from flowers of Rhododendron mucronatum.@Zhong Yao Cai., 36, 1617-1619.@Yes$Hall R., Beale M., Fiehn O., Hardy N., Sumner L. and Bino R. (2002).@Plant metabolomics: the missing link in functional genomics strategies.@Plant Cell, 14, 1437-1440.@Yes$Bodoprost H. and Rosemeyer J. (2007).@Analysis of phenacylester Derivatives of fatty acids from, Human skin surface Sebum by RP-HPLC: Chromatograpic mobility as a function of physiochemical properties.@International Journal of molecular sciences, 8(11), 1111-1124.@Yes$Lewis K. and Ausubel F.M. (2006).@Prospects for plant-derived antibacterials.@Nat Biotech., 24(12), 1504-1507.@Yes$Adekunle A.S. and Adekunle O.C. (2009).@Preliminary assessment of antimicrobial properties of aqueous extract of plants against infectious diseases.@Biol Med., 1(3), 20-24.@Yes$Olagunju J.A., Fagbohunka B.S., Oyedapo O.O. and Abdul A.I.A. (2006).@Effects of an ethanolic root extract of Plumbago zeylanica L on some serum parameters of the rats.@RPMP-Drug Dev Mol., 11, 268-276.@Yes