International Science Community Association International Research Journal of Biological Sciences 2278-3202 8 2 2019 02 10 Seasonal variation in bacterial diversity of Tuva Timba thermal springs of Gujarat, India 6 14 EN Sadanandan Sruthy 1 Department of Zoology, S. B College, Changanassery-686101, Kerala, India and TIES, Ecological Research Campus, K.K.Road, Vellore P.O., Kottayam-686 501, Kerala, India and Abad food servics, Abad Fisheries Pvt. Ltd, Kochangadi, Chullickal, Kochi-682202, Kerala, India M.V. Vincy 2 Department of Zoology, S. B College, Changanassery-686101, Kerala, India Elias Roshni Susan 3 TIES, Ecological Research Campus, K.K.Road, Vellore P.O., Kottayam-686 501, Kerala, India Prasad Chandhu K. 4 Department of Zoology, S. B College, Changanassery-686101, Kerala, India and TIES, Ecological Research Campus, K.K.Road, Vellore P.O., Kottayam-686 501, Kerala, India George Jessen 5 Eben-Ezer Degree College, Eben-Ezer Group of Institutions, No.19, Hennur-Bangalur Main Road, Bangalore, Karnataka, India Patel Disha N. 1 Department of Life Sciences, Gujarat University, Ahmedabad, India and Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India Dave Shailesh R. 2 Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India Braganza Vincent J. 3 Xavier Research Foundation, Loyola Centre for Research & Development, Ahmedabad, India Modi Hasmukh A. 4 Department of Life Sciences, Gujarat University, Ahmedabad, India > Research Paper 2018 10 9 2019 02 10 The seasonal bacterial diversity study of thermophilic isolated from TuvaTimba hot springs in terms of morphological, physicochemical, biochemical tests and molecular characterization. A total of 48 isolates were cultivated using various media. Out of these isolates 25 were Gram-positive, 19 Gram-negative and 4 were Gram variable. Based on the metabolic activity of these isolates, 29 could produce catalase, 45, 47 and 24 utilized casein, starch, and citrate respectively. 22 produced H2S by sulphate reduction. Sugar utilization varied in all the isolates. Diversity indices such as Simpson, Shanon, Menhinick and Margalef index were also studied based on their metabolic activity. 25 well isolated purified colonies were selected for molecular analysis. Based on this study the identified isolates belonged to genera Bacillus, Brevibacillus, Geobacillus, Anoxybacillus and Brevibacterium. All these isolates may have potential biotechnological and industrial application. Copyright@ International Science Community Association