International Science Community Association International Research Journal of Biological Sciences 2278-3202 8 10 2019 10 10 Phytochemical analysis, antioxidant, antimicrobial, and antiurolithiasis activities of endophytic penicillium notatum isolated from aloe vera roots 31 37 EN Pradeep Kumar 1 Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, India Arti Choudhary 2 Transport Planning and Environment Division, CSIR-Central Road Research Institute, New Delhi, India Rajendra Prasad 3 Department of Physics, Indian Institute of Technology (BHU), Varanasi, India Dileep Kumar Gupta 4 Department of Physics, Indian Institute of Technology (BHU), Varanasi, India Meenakshi Amarawat 5 Department of Botany, Bhupal Noble′s University Udaipur, India Gaurav Shukla 6 Department of Civil Engineering, Indian Institute of Technology Roorkee, India Abhay Kumar Singh 7 Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, India Meenakshi Amarawat 1 Department of Botany, Bhupal Noble′s University Udaipur 313001, India Pradeep Kumar 2 Department of Physics, Institute of Science, Banaras Hindu University Varanasi 221005, India M.S. Rathore 3 Department of Botany, Bhupal Noble′s University Udaipur 313001, India Omorodion Nnenna 1 Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigrria Nwala Chinyere 2 Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigrria Sonali Sasmal 1 Department of Chemistry, School of Applied Sciences, Centurion University of Technology and Management, Odisha, India Arun Kumar Pradhan 2 Department of Chemistry, School of Applied Sciences, Centurion University of Technology and Management, Odisha, India Susanta Kumar Biswal 3 Department of Chemistry, School of Applied Sciences, Centurion University of Technology and Management, Odisha, India Atia Arzoo 4 Department of Environmental Science, School of Applied Sciences, Centurion University of Technology and Management, Odisha, India Rohit S. Mane 1 Department of Microbiology and Biotechnology, Karnatak University, Dharwad, Karnataka, India and Department of Life Sciences, Mandsaur University, Mandsaur, M.P. India Priyanka R. Jagtap 2 Department of Microbiology, New arts, Commerce and Science College, Ahmednagar, Maharashtra, India Manoj A. Vyawahare 3 Department of Microbiology, New arts, Commerce and Science College, Ahmednagar, Maharashtra, India Amarja H. Bhosale 4 Department of Microbiology, Dr. Babasaheb Ambedkar Marathawada University, Sub-Campus Osmanabad, Maharashtra, India Vishrut V. Ghare 5 Department of Microbiology, S.B.B Alias Appasaheb Jedhe College of Arts, Commerce & Science, Pune, India Bidhayak Chakraborty 6 Department of Botany, Karnatak University, Dharwad, Karnataka > Research Paper 2019 4 29 2019 10 10 The objective of present investigation was to assess phytochemical analysis, antioxidant, antimicrobial, and antiurolithiasis activities of endophytic fungi derived bioactive compounds obtained from Aloe vera roots. Fungal endophytes were isolated by means of potato dextrose agar media from Aloe vera roots and grown in Potato dextrose broth for the production of the bioactive compounds. The qualitative phytochemical screening was performed for the aqueous crude extract of the fungus. Phytochemicals like phenols and flavonoids were quantitatively estimated and further purified by thin layer chromatography and FTIR. The antiurolithiasis and antimicrobial activity of crude extracts was analyzed. A whole of six fungal endophytes were isolated and Penicillium notatum were identified as core endophytic fungus by 18S rRNA analysis. The fungus revealed total 1.86g and 0.15g of wet and dried biomass in PDB/100ml with 2.60g/100ml of the aqueous crude extract. The phytochemical screening showed terpenoids, flavonoids, saponins, phenols and tannins, alkaloid, cardiac glycosides, fixed oils and fats, proteins, carbohydrates with 2.26g of phenol and 0.22g of flavonoids/100g of gallic acid equivalent quantitatively. TLC revealed intermediate polar basic compounds, polar basic compounds, alkaloids, flavonoids, saponins, and terpenoids while FTIR analysis documented different functional groups such as alkanes, amides, alkenes, alcohol and phenols, alkyl halides, ether, amines, and nitrile groups. Ferric ions reducing power was reported at the rate of 1.2407±0.00702 while PM assays reported 0.8983±0.00351. The crude extract showed highest inhibition zone at the 100% concentration against Escherichia coli (16.3±0.57) and Aspergillus niger (20.5± 0.24). The maximum antiurolithiasis activity showed at 100% concentration at the rate of 67.5±1.49 of crude extract. Copyright@ International Science Community Association