@Research Paper
<#LINE#>Isolation of Indole acetic acid producing bacteria from digester effluent and their effect on plant growth promotion<#LINE#>V.S. @Patil <#LINE#>1-9<#LINE#>1.ISCA-IRJBS-2018-044.pdf<#LINE#>Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India<#LINE#>8/7/2018<#LINE#>20/12/2018<#LINE#>The synthesis and release of indole acetic acid is an important property of bacteria that play key role in stimulating growth of crops. Indole acetic acid is phytohormone involved in growth and development in plants. The study aimed for isolation and to identify IAA producing bacteria from digester effluent of vegetable waste based biomethanation plant and to test its growth stimulatory effect on crops. Seventeen bacteria were isolated from digester effluent. They were tested to determine production ability for IAA. Two most potent IAA producing bacteria were selected further to test its stimulatory effect on growth of crops by pot assay method. The potent IAA producing plant growth promoting bacterial isolates were identified by molecular characterization using 16 S rRNA gene analyses. The results obtained from pot experiment demonstrated that two most potent IAA producing bacterial isolates cause significant increase in plant development parameters of inoculated crop plants by comparing with control. The study suggests two potent IAA producing bacteria from digester effluent can serve as efficient biofertilizer inoculants to enhance soil fertility and plant growth promotion.<#LINE#>Matsukawa E., Nakagawa Y., Iimura Y. and Hayakawa M. (2007).@Stimulatory effect of indole-3- acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp.@Actinomycetologica, 21, 32-39.@Yes$Carreno-Lopez R., Campos-Reales N., Elmerich C. and Baca B.E. (2000).@Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense.@Mol. Gen. Genet., 264(4), 521-530.@Yes$Spaepen S., Vanderleyden J. and Remans R. (2007).@Indole-3-acetic acid in microbial and microorganism-plant signaling.@FEMS Microbiol. Rev., 31(4), 425-448.@Yes$Persello-Cartieaux F., Nussaume L. and Robaglia C. (2003).@Tales from the underground: molecular plant-rhizobacteria interactions.@Plant Cell Environ., 26(2), 189-199.@Yes$Zhao Y. (2010).@Auxin biosynthesis and its role in plant development.@Annu. Rev. Plant Biol., 61, 49-64.@Yes$Teale W.D., Paponov I.A. and Palme K. (2006).@Auxin in action: signaling, transport and the control of plant growth and development.@Nat. Rev. Mol. Cell Biol., 7(11), 847- 859.@Yes$Swain M.R., Naskar S.K. and Ray R.C. (2007).@Indole 3-acetic acid production and effect on sprouting of yam, (Dioscorea rotundata L) Minisetts by Bacillus subtilis isolated from culturable cowdung microflora.@Pol. J. Microbiol., 56(2), 103-110.@Yes$Pattern C.L. and Glick B.R. (2002).@Role of Pseudomanas putida indo lactic acid in development of the host plant root system.@Appl. Environ. Microbiol., 68(8), 3795-3801.@Yes$Harikrishnan H., Shanmugaiah V. and Balasubramanian N. (2014).@Optimization for production of Indole acetic acid (IAA) by plant growth promoting Streptomyces sp VSMGT1014 isolated from rice rhizosphere.@Int. J. Curr. Microbiol. App. Sci., 3(8), 158-171.@Yes$Pant G. and Agrawal P.K. (2014).@Isolation and characterization of indole acetic acid producing plant growth promoting rhizobacteria from rhizospheric soil of Withania somnifera.@Journal of Biological and Scientific Opinion, 2(6), 377-383.@Yes$Owamah H.I., Dahunusi S.O., Oranusi U.S. and Alfa M.I. (2014).@Fertilizer and sanitary quality of digestate biofertilizer from co-digestion of food waste and human excreta.@Waste manag., 34(4), 747-752.@Yes$Hassan D.U. and Abdulsalam S. (2017).@Assessement of bio-fertilizer quality of anaerobic digestion of watermelon peels and cow dung.@Chemical and Biomolecular Engineering, 2(3), 135-141.@Yes$Alfa M.I., Adie D.B., Igboro S.B., Oranusi U.S., Dahunsi S.O. and Akali D.M. (2014).@Assessment of biofertilizer quality and health implications of anaerobic digestion effluent of cow dung and chicken droppings.@Renewable Energy, 63, 681-686.@Yes$Report (2008).@Bio-fertilizer Entrepreneurial training manual.@Tamil Nadu Agricultural University, Coimbatore, India.@No$Rahman A., Sitepu I.R., Tang S.Y. and Hashidoko Y. (2010).@Salkowski's reagent test as a primary screening index for functionalities of Rhizobacteria isolated from wild dipterocarp saplings growing naturally on medium-strongly acidic tropical peat soil.@Biosci. Biotechnol. Biochem., 74(11), 2202-2208.@Yes$Gordon S.A. and Weber R.P. (1951).@Colorimetric estimation of indoleacetic acid.@Plant Physiol., 26(1), 192-195.@Yes$Chung K.R., Shilts T., Erturk U., Timmer L.W. and Uenq P.P. (2003).@Indole derivatives produced by the fungus Colletotrichum acutatum causing lime anthracnose and postbloom fruit drop of citrus.@FEMS Microbiol. Lett., 226(1), 23-30.@Yes$Sujatha N. and Ammani K. (2013).@Siderphore production by the isolates of fluorescent pseudomonads.@Int. J. Cur. Res. Rev., 5(20), 1-7.@Yes$Cappuccino J. and Sherman N. (2010).@Microbiology: A Laboratory Manual.@9th edition, Benjamin Cummings Publishing Company, USA.@No$Alstrom S. and Burns R.G. (1989).@Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition.@Biol. Fertil. Soils, 7(3), 232-238.@Yes$Vos P., Garrity G., Jones D., Krieg N.R., Ludwig W., Rainey F.A., Schleifer K.H. and Whitman W.B. (2009).@Bergey@The Firmicutes, 2nd Edition, Springer Dordrecht Heidelberg London, New York, 3, 1-1422.@No$Brenner D.J., Krieg N.R. and Staley J.T. (2005).@Bergey@Part C, Second Edition, Springer, 2, 308-316.@Yes$Wahyudi A.T., Astuti R.P., Widyawati A., Meryandini A. and Nawangsih A.A. (2011).@Characterization of Bacillus sp. strains isolated from rhizosphere of soybean plants for their use as potential plant growth for promoting Rhizobacteria.@J.Microbiol. Antimicrob., 3(2), 34-40.@Yes$Sridevi M. and Mallaiah K.V. (2007).@Bioproduction of indole acetic acid by rhizobium strains isolated from root nodules of green manure crop.@Sesbania sesban (L.) Merr. Iranian J. Biotech., 5(3), 178-182.@Yes$Lwin K.M., Myint M.M., Tar T. and Aung W.Z.M. (2012).@Isolation of plant hormone (indole-3-acetic acid-IAA) producing rhizobacteria and study on their effects on maize seedling.@Engineering Journal, 16(5), 137-144.@Yes$Ahmad F., Ahmad I. and Khan M.S. (2005).@Indole Acetic Acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of Tryptophan.@Turk J. Biol., 29, 29-34.@Yes$Malik D.K. and Sindhu S.S. (2011).@Production of indole acetic acid by Pseudomonas sp.: effect of coinoculation with Mesorhizobium sp. Cicer on nodulation and plant growth of chickpea (Cicer arietinum).@Physiol. Mol. Biol. Plants, 17(1), 25-32.@Yes$Fatima Z., Saleemi M., Zia M., Sultan T., Aslam M., Rehman R. and Chaudhary M.F. (2009).@Antifungal activity of plant growth promoting rhizobacteria isolates against Rhizoctonia solani in wheat.@Afr. J. Biotechnol., 8(2), 219-225.@Yes$Xu Z., Shao J., Li B., Yan X., Shen Q. and Zhang R. (2013).@Contribution of Bacillomycin D in Bacillus amyloliquefaciens SQR9 to antifungal activity and biofilm formation.@Appl. Environ. Microbiol., 79(3), 808- 815.@Yes$Pidello A. (2003).@The effect of Pseudomonas fluorescens strains varying in psoverdine production on the soil redox status.@Plant and soil, 253(2), 373-379.@Yes
<#LINE#>Hydrocarbon degradation potential of some hydrocarbon-utilizing bacterial species associated with Kenaf (Hibiscus cannabinus L.) plant<#LINE#>Ashikodi @A.O.,Abu @G.O. <#LINE#>10-19<#LINE#>2.ISCA-IRJBS-2018-075.pdf<#LINE#>Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigeria@Department of Microbiology, University of Port Harcourt, P.M.B 5323, Port Harcourt, Rivers State, Nigeria<#LINE#>29/8/2018<#LINE#>15/12/2018<#LINE#>13 Kenaf (Hibiscus cannabinus) plant associated Hydrocarbon-Utilizing Bacterial isolates previously identified based on their morphological, biochemical and molecular characteristics as Providencia vermicola_01, Providencia rettgeriB10_04, Providencia rettgeri D04_10,Pseudomonas aeruginosa D10_10, Exiguobacterium aurantiacum F03_18, Providencia vermicola F10_16, Klebsiella pneumoniae G10_19, Stenotrophomonas maltophila  A04_01, Providencia rustigianii H10_22, Lysinibacillus fusiformis B11_05, Lysinibacillus sphaericus C11_08, Pseudomonas aeruginosa F04_16  and Lysinibacillus sphaericus E11_were  investigated for their degradation potential in crude oil, diesel and engine oil. The Kenaf (Hibiscus cannabinus) plant was grown in a Niger Delta soil.  Growth of these Kenaf associated bacteria was assessed for 7 days by monitoring the optical density (OD) of the media. OD values were observed to rise majorly between the first and three days of contact between the HUB and the hydrocarbons. The highest OD values measured for crude oil degradation was 1.548nm by Klebsiella pneumoniae G10_19, for engine oil 1.416nm by Klebsiella pneumonia and by Exiguobacterium aurantiacum 1.416nm. Significant difference in o.d. values was observed only for Exiguobacterium aurantiacum F03_18. This study provided information on suitable Kenaf (Hibiscus cannabinus) HUB bacterial species to use for phytoremediation of engine oil, crude oil and diesel impacted soil in Niger Delta Region.<#LINE#>Vieira P.A., Vieira R.B., De Fran&ccedil;a F.P. and Cardoso V.L. (2007).@Biodegradation of effluent contaminated with diesel fuel and gasoline.@Journal of Hazardous Materials, 140(1-2), 52-59.@Yes$Romantschuck M., Sarand I., Pet&auml;nen T., Peltola R., Johnsson&#8208;Vihanne M., Koivula T., Yrjala K. and Haahtela K. (2000).@Means to Improve the Effect of In situ Bioremediation of Contaminated Soil: An Overview of Novel Approaches.@Envir. Pol, 107(2), 179&#8208;185.@Yes$Chaillan F., Le Fl&egrave;che A., Bury E., Phantavong Y.H., Grimont P., Saliot A. and Oudot J. (2004).@Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms.@Research in microbiology, 155(7), 587-595.@Yes$Agwu M.O. (2013).@Community Participation and Sustainable Development in the Niger Delta.@Brit. J. of Edu. Soc and Beha. Sci., 3, 33-46.@No$Oviasuyi P.O. and Uwadiae J. (2010).@The Dilemma of Niger Delta Region as Oil Producing States of Nigeria.@J. of P. Con. and. Dev, 16, 110-126.@Yes$Olanrewaju J.S. (2015).@The Issues of Integration and Marginalization in a Federation: South-South Question in Nigerian Politics.@Int. J. of Pol and G. Gov., 6, 1-16.@Yes$Agbonifo P. (2016).@Oil Spills Injustices in the Niger Delta region: Reflections on Oil Industry Failure in Relation to the United Nations Environment Programme (UNEP) REPORT.@Int J. of Pet and Gas. Exp. Mgt, 2, 26-37.@Yes$Karthikeyan R. and Kulakow P.A. (2003).@Soil Plant Microbe Interactions in Phytoremediation.@Adv. Bio. Eng. Bio, 78, 52-74.@Yes$Okoh A.I. (2003).@Biodegradation of Bonny light Crude Oil in Soil Microcosm by some Bacterial Strains Isolated from Crude Oil Flow Stations Saver Pits in Nigeria.@Afr. J. of Bio, 2(5), 104-108.@Yes$Adeline S.Y., Ting C. and Aw C.S. (2009).@Hydrocarbon-degradation by isolate Pseudomonas lundensis UTAR FPE2.@Malaysian Journal of Microbiology, 5(2), 104-108.@Yes$Abu G.O. and Ogiji P.A. (1996).@Initial test of a bioremediation scheme for the cleanup of an oil-polluted waterbody in a rural community in Nigeria.@Bioresource Technology, 58(1), 7-12.@Yes$Chikere C.B. and Ekwuabu C.B. (2014).@Molecular characterization of autochthonous hydrocarbon utilizing bacteria in oil-polluted sites at Bodo Community, Ogoni land, Niger Delta, Nigeria.@Nig. J. of Bio, 27, 28-33.@Yes$Ikuesan F.A., Boboye B.E. and Adetuyi F.C. (2015).@Cow dung as inoculum carrier for the degradation of crude oil.@Sky Journal of Microbiology Research, 3(4), 47-54.@Yes$Jeswani H. and Mukherji S. (2013).@Batch Studies with Exiguobacterium aurantiacum Degrading Structurally Diverse Organic Compounds and its Potential For Treatment of Biomass Gasification Waste Water.@Int.  Bio. and Bio, 80, 1-9.@Yes$Mohanty G. and Mukherji S. (2008).@Biodegradation rate of diesel range n-alkanes by bacterial cultures Exiguobacterium aurantiacum and Burkholderia cepacia.@International Biodeterioration & Biodegradation, 61(3), 240-250.@Yes$Moneke A. and Nwangwu C. (2011).@Studies on the bioutilization of some petroleum hydrocarbons by single and mixed cultures of some bacterial species.@African Journal of Microbiology Research, 5(12), 1457-1466.@Yes
@Short Communication
<#LINE#>Degradation of feathers by bacterial consortium and its application in seed germination<#LINE#>Parimita @Jadhav,Girish @Pathade <#LINE#>20-23<#LINE#>3.ISCA-IRJBS-2018-056.pdf<#LINE#>Department of Microbiology, Shardabai Pawar Mahila Mahavidyalaya, Shardanagar Baramati, Maharashtra, India@Department of Microbiology, H.V. Desai College, Pune, Maharashtra, India<#LINE#>30/7/2018<#LINE#>28/12/2018<#LINE#>Feathers primarily composed of keratin are largely produced as a waste by-product at poultry plants. Keratin is an insoluble protein macromolecule with very high stability and low degradation rate. Use of keratinolytic bacteria have found a promising effect in shortening the degradation time. In present study consortium of three organisms was used for feather degradation and the feather degraded product was used to check effect on seed germination. It was seen that the consortium was able to degrade 92.6% of feathers within 72 hours. The degraded product proved to be rich source of nitrogen. The application of feather degraded product in seed germination of Gram seeds had astounding results. The effect was measured in terms of root-shoot length, number of secondary roots, root shoot ratio, etc. The root: shoot ratio was increased in control group (2.7) compared with test (1.9) indicating nutrient deficiency. Thus, the feather degraded product could be used to increase seed germination rate.<#LINE#>Vasileva-Tonkova E., Gousterova A. and Neshev G. (2009).@Ecologically safe method for improved feather wastes biodegradation.@International Biodeterioration and Biodegradation, 63(8), 1008-1012.@Yes$Brandelli A., Daroit D.J. and Riffel A. (2010).@Biochemical features of microbial keratinases and their production and applications.@Applied Microbiology and Biotechnology, 85(6), 1735-1750.@Yes$Deivasigamani B. and Alagappan K.M. (2008).@Industrial application of keratinase and soluble proteins from feather keratins.@Journal of Environmental Biology, 29(6), 933-936.@Yes$Papadopoulos M.C., El Boushy A.R., Roobdeen A.E. and Ketelaars E.H. (1986).@Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal.@Anim. Feed Sci. Technol., 14(3-4), 279-290.@Yes$Onifade A.A., Al-Sane N.A., Al-Musallam A.A. and Al-Zarban S. (1998).@A review: Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources.@Bioresource Technology, 66, 1-11.@Yes$Selvam K. and Vishnupriya B. (2012).@Biochemical and molecular characterization of microbial keratinase and its Remarkable Applications.@International Journal of Pharmaceutical and Biological Archieves, 3(2), 267-275.@Yes$Choi J.M. and Nelson P.V. (1996).@Developing a slow-release nitrogen fertilizer from organic sources using poultry feathers.@Journal of the American Society of Horticultural Science, 121(4), 634-638.@Yes$Kim W.K., Lorenz E.S. and Patterson E.S. (2002).@Effect of Enzymatic and Chemical Treatments on Feather Solubility and Digestibility.@Poult. Sci., 81, 95-98.@Yes$Paul T., Halder S.K., Das A., Bera S., Maity C., Mandal A. and Mondal K.C. (2013).@Exploitation of chicken feather waste as a plant growth promoting agent using keratinase producing novel isolate Paenibacillus woosongensis TKB2.@Biocatalysis and Agricultural Biotechnology, 2(1), 50-57.@Yes$Anwar M.S., Siddique M.T., Verma A., Rao Y. R., Nailwal T., Ansari M. and Pande V. (2014).@Multitrait plant growth promoting (PGP) rhizobacterial isolates from Brassica juncea rhizosphere: keratin degradation and growth promotion.@Communicative & integrative biology, 7(1), e27683.@Yes$Kornillowicz-Kowalska T. and Bohacz J. (2011).@Biodegradation of keratin waste: Theory and practical aspects.@Waste Management, 31(8), 1689-1701.@Yes$Vasileva-Tonkova E., Gousterova A. and Neshev G. (2009).@Ecologically safe method for improved feather wastes biodegradation.@International Biodeterioration & Biodegradation, 63(8), 1008-1012.@Yes$Ichida J.M., Krizova L., LeFevre C.A., Keener H.M., Elwell D.L. and Burtt Jr, E.H. (2001).@Bacterial inoculum enhances keratin degradation and biofilm formation in poultry compost.@Journal of Microbiological Methods, 47(2), 199-208.@Yes$Maerere A.P., Kimbi G.G. and Nonga D.L.M. (2001).@Comparative effectiveness of animal manures on soil chemical properties, yield and root growth of amaranthus (Amaranthus cruentus L.).@African Journal of Science and Technology, 1(4), 14-21.@Yes$Megan B. (2013).@Education.com@updated on Oct 25, 2013.@Yes
<#LINE#>A study of haemogram of mud Eel Monopterus cuchia (Ham.) from Eastern Himalayas, India<#LINE#>Prasanta @Nanda,B. @Dabi,L. @Wangchu,D. @Narba,Hiranmaya @Sharma <#LINE#>24-26<#LINE#>4.ISCA-IRJBS-2018-073.pdf<#LINE#>Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India@Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India@Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India@Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India@Department of Zoology, Dera Natung Government College, Itanagar, Arunachal Pradesh-791113, India<#LINE#>16/9/2018<#LINE#>8/12/2018<#LINE#>Monopterus cuchia is a common mud eel distributed all along the North-Eastern India and Bangladesh. The high survival rates of this fish during transportation and marketing, along with its economic value makes it a highly demanding fish in the North-Eastern part of India. M. cuchia is known to have high erythrocytes indices. Hence the present work was carried out to study the morphometric index and its co-relation with the erythrocytic parameter of fish M. cuchia (Hamilton) from Eastern Himalayas. Blood parameters like haemoglobin content (Hb%), total erythrocyte count (TECX106 mm-3) and packed cell volume (PCV %) were done. Absolute values like mean cell haemoglobin (MCH), mean cell haemoglobin concentration (MCHC) and mean cell volume (MCV) were done from the above blood parameters and data were correlated(r). The results indicates that there is a positive correlation (r=+0.85) between length and weight of the fish. When the Hb content was compared with TEC, it was positively correlated (r=+0.96). However, there was no correlation of haemoglobin either with length (r=+0.08) or weight (r=-0.03).<#LINE#>Miah M.F., Naser M.N. and Ahmed M.K. (2015).@The Fresh Water Mud Eel, Monopterus cuchia-A Review.@Journal of Global Biosciences, 4(3), 1780-1794.@Yes$Baruah I., Goswami U.C., Borah B.C. and Bhuyan S. (2013).@Effect of sublethal concentration of malathion on hematological parameters of Monopterus cuchia (Hamilton-Buchanan).@Bioscan, 8(3), 1111-1114.@Yes$Nanda P. and Panigrahi S. (2016).@Haematological alteration in air breathing fishes on paper mill effluent.@Biolife, 4(4), 704-706.@No$Mishra N., Pandey P.K., Datta Munshi J.S. and Singh B.R. (1977).@Haematological parameters of an air&#8208;breathing mud eel, Amphipnous cuchia (Ham.)(Amphipnoidae; Pisces).@Journal of Fish Biology, 10(6), 567-573.@Yes$Dacie J.V. and Lewis S.M. (1977).@Practical Haematology.@J & A Churchill Ltd. London, U.K.@No$Nanda P. (1997).@Haematological changes in common Indian catfish Hetropneustes fossilis under Nickel stress.@J. Ecobiol., 9(4), 243-246.@Yes$Dube S.C. and Munshi J.S. (1973).@The quantitative study of erythrocytes and haemoglobin in the blood of an air breathing fish Anabas testudineus in relation to its body size.@Folia haematol, 100(4), 436-446.@Yes$De Souza P.C. and Bonilla-Rodriguez G.O. (2007).@Fish hemoglobins.@Brazilian Journal of Medical and Biological Research, 40(6), 769-778.@Yes$Krogh A. and Leitch I. (1919).@The respiratory function of blood of fishes.@J. Physiol. London, 52(5), 288-300.@Yes$Hughes G.M. and Datta munshi J.S. (1973).@Nature of air breathing organs of Indian Fishes Channa, Amphioxus, Clarias and Saccobranch as shown by Electron Microscopy.@J. Zool, 170(2), 245-270.@Yes$Glomski C.A., Tamberlin J. and Chainani M. (1992).@The phylogenetic odyssey of the erythrocyte. III. Fish, the lower vertebrate experience.@Histol.Histopath., 7(3), 501-528.@Yes$Affonso E.G., Val A.L. and de Almeida V.M.F. (1990).@Adaptative features of Amazon fishes. Hemoglobins, hematology, intraerythrocytic phosphates and whole blood Bohr effect of Pterygoplichthys multiradiatus.@Comp. Biochem. Physiol.B., 97, 435-440.@Yes$Farmer M. (1979).@The transition from water to air breathing: effects of CO2 on hemoglobin function.@Comparative Biochemistry and Physiology Part A: Physiology, 62(1), 109-114.@Yes$Dubale M.S. (1963).@Aerial respiration in fishes: a review.@Proc. Fast summer school of zoology (Shimla, 1961), 325-337.@Yes$Ramaswamy M. and Reddy T.G. (1978).@A comparative study of haematology of three air breathing fishes.@Proc. Indian Acad. Sci., 87(12), 381-385.@Yes
<#LINE#>A comprehensive study on relationship between Euploea core and Nerium indicum present in Fergusson College, Pune, India<#LINE#>Sameer @Terdalkar,Snehangshu @Das,Priyanka @Patil,Minakshi @Mahajan <#LINE#>27-31<#LINE#>5.ISCA-IRJBS-2018-074.pdf<#LINE#>Department of Zoology, Fergusson College, Pune-411004, Maharashtra, India@Department of Botany, Shivaji University, Kolhapur-416004, Maharashtra, India@Department of Botany, Fergusson College, Pune-411004, Maharashtra, India@Department of Botany, Fergusson College, Pune-411004, Maharashtra, India<#LINE#>15/9/2018<#LINE#>23/12/2018<#LINE#>The life cycle of Danaidae butterfly Euploea core core Cramer (Lepidoptera: Rhopalocera: Danaidae) (Common Indian Crow) and its larval association with one of its known host plant, Nerium indicum (Gentianales: Apocynaceae), was investigated at Fergusson College campus by an effortful scientific observational approach by keeping them intact in their "chosen barrier free" natural habitat. Three particular sites was chosen where matured plants of N. indicum were already present. The morphological and detectable behavioural aspects of fourth and fifth instar larvae, pupae and the emergence of adult out of its pupal stage were observed in the selected study site at temperature ranging from 26&deg;C-28.7&deg;C. This study presents a pilot approach towards ovipositional behaviour of adult E. core in choosing a suitable site for laying eggs in the natural environment by studying the height, coordinates, daily weather conditions and neighbouring environmental factors. In addition to the lifecycle, the current study also adds a note on the larval food relationship with N. indicum by focusing on the sequestrational power of larvae of E. core to the harmful cardiac glycosides present as a defense mechanism in the foliage of Indian Oleander.<#LINE#>Chandar G.S., Rao K.E. and Atluri J.B. (2016).@Metamorphosis of Euploea core (Common Crow) on Nerium indicum (Mill.) at Andhra University Campus, Visakhapatnam.@World Journal of Pharmacy and Pharmaceutical Sciences, 5(9), 1435-1450.@Yes$New T.R., Pyle R.M., Thomas J.A., Thomas C.D. and Hammond P.C. (1995).@Butterfly conservation management.@Annual review of entomology, 40(1), 57-83. https://doi.org/10.1146/annurev.en.40.010195.000421@Yes$Smetacek P. (1996).@Restoring past glory.@Santuary Asia, 16(6), 26-29.@Yes$Vartak V.D. (1958).@The flora of the Fergusson College campus, Poona dist.@Fergusson College Magazine, 50(2), 7-11.@No$Nerlekar A.N., Lapalikar S.A., Onkar A.A., Laware S.L. and Mahajan M.C. (2016).@Flora of Fergusson College campus, Pune, India: monitoring changes over half a century.@Journal of Threatened Taxa, 8(2), 8452-8487. http://dx.doi.org/10.11609/jott.1950.8.2.@Yes$Nerlekar A.N., Gowande G.G. and Joshi P.S. (2014).@Diet of the Spotted Owlet Athene brama in an urban landscape.@Indian BIRDS, 9(2), 45-48.@No$Nerlekar A.N., Gowande G.G. and Joshi P.S. (2014).@Behavioural ethogram of Spotted Owlet Athene brama (Temminck, 1821).@Journal of the Bombay Natural History Society, 111(3), 172-179. http://dx.doi.org/ 10.17087/jbnhs/2014/v111i3/82356@Yes$Nerlekar A.N., Warudkar A.M., Gowande G.G., Salve S. S., Raut A., Patankar S.R. and Nalavade S.B. (2016).@A review of the faunal diversity of the Fergusson College campus, Pune, India.@ZOO's PRINT, 31(10), 4-25.@Yes$Kumar N. (1984).@A Checklist of the butterflies of Fergusson College.@Fergusson College Magazine, 75, 14-17.@No$Chhaya K., Mujumdar N., Mhaske P. and Patwardhan A. (2012).@A new larval host record for the Pea Blue butterfly Lampides boeticus (Linnaeus) (Insecta: Lepidoptera: Lycaenidae) from Pune, Maharashtra, India.@Bugs R ALL Newsletter of the Invertebrate Conservation & Information Network of South Asia, 19, 6-9.@Yes$Warudkar A. and Patankar S. (2013).@Butterflies in Fergusson College.@Fergusson College Magazine, 104, 3-4.@No$Shiojiri K., Sabelis M. and Takabayashi J. (2015).@Oviposition preference of cabbage white butterflies in the framework of costs and benefits of interspecific herbivore associations.@Royal Society open science, 2(12), 150524.@Yes$Saikia K., Kalita J. and Saikia P.K. (2010).@Biology and life cycle generations of common crow-Euploea core core Cramer (Lepidoptera: Danainae) on Hemidesmus indica host plant.@Int J NeBIO, 1(3), 28-37.@Yes$Wynter-Blyth M.A. (1957).@Butterflies of the Indian region.@Today and Tomorrow@No$Varshney R.K. (1993).@Index Rhopalocera Indica Part III. 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