International Science Community Association International Research Journal of Biological Sciences 2278-3202 7 5 2018 05 10 Physico-chemical and microbiological analysis of Ikpoba River Water in Benin City, Edo State, Nigeria 1 6 EN Akatah Hilda Abiola 1 Department of Biological Sciences, Faculty of Natural and Applied Sciences, Anchor University, Lagos, Nigeria Omonigho Solomon 2 Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria Ibrahim Azeez God\'s Gift 3 Department of Biological Sciences, Faculty of Natural and Applied Sciences, Anchor University, Lagos, Nigeria Seyinde Doris Onolenmen 4 Department of Chemical Sciences, Faculty of Natural and Applied Sciences, Anchor University, Lagos, Nigeria > Research Paper 2018 1 10 2018 05 10 Surface waters are always polluted because they are exposed directly to contaminants and therefore do not usually meet required standards. The physico-chemical and microbiological analyses of Ikpoba River was examined to determine the specie composition, microbial load and the antibiotic susceptibility pattern of the isolated strains. Analysis of the physicochemical parameter shows that all except manganese and iron were within WHO permissible limit. The total microbial population counts ranged from 3.3x103 to 6.7x103 CFU/ml and 4.75x104 to 9.7x104 CFU/ml for the fungal and bacterial isolates respectively. Total coliform counts spans from 434 MPN/100 ml to 819 MPN/100 ml. The microorganisms isolated were Aspergillus flavus, Geotrichum sp, Penicillium sp., Aspergillus niger, Mucor sp., Aspergillus fumigatus, Trichoderma sp., Escherichia coli, Staphylococcus aureus, Salmonella sp., Pseudomonas sp., Shigella sp., Vibrio sp., Proteus sp., Bacillus sp and Klebsiella sp.. The percentage of resistance of the antibiotics ranged from amoxicillin (20%) to ciprofloxacin (5%). The variations in the bacteriological and physicochemical parameters of various stations of the river water samples when compared had no significant difference (P>0.05). Therefore, Ikpoba river water is contaminated with substances which may be harmful to human health. Copyright@ International Science Community Association