International Science Community Association International Research Journal of Biological Sciences 2278-3202 7 3 2018 03 10 Transcriptomic analysis of Agelenopsis naevia (Aranae: Agelenidae) Venom Gland 9 23 EN Alakesh Barman 1 P.G. Department of Zoology, Bajali College, Barpeta-781325, India Binode Kr. Baruah 2 Department of Zoology, Cotton University, Guwahati-781001, India Ahmed J. 1 Department of Zoology, Ahmadu Bello University Zaria, Nigeria Shehu D.M. 2 Department of Zoology, Ahmadu Bello University Zaria, Nigeria Ndams I.S. 3 Department of Zoology, Ahmadu Bello University Zaria, Nigeria > Research Paper 2017 11 28 2018 03 10 The venom of spider is made up of toxins with varying biological activities whose selectivity and affinity for various receptors and ion channels are yet to be sufficiently explored and exploited. Recently, transcriptomics have been employed as a tool to reveal the molecular diversity and structure of animal venoms across species. Thus, this study was carried out to determine the transcripts coding for toxins in the venom gland of Agelenopsis naevia, collected in open gardens of Ahmadu Bello University Zaria, Nigeria. Venom glands were isolated using micro dissection followed by mRNA extraction. A cDNA library was constructed and pair-end sequencing was carried out. A total of 11,167,123 reads were generated which were assembled into 33,182 sequences. Fourty eight (48) transcript coded for proteins/peptides amongst which are sphingomyelinase-D, hyaluronidase, astacin-like metalloproteases, techylectin and cystine knot toxins. The results provide insight towards the discovery of novel potential bioinsecticides and/or drug leads from Agelenopsis Naevia venom for agro-allied/pharmaceutical applications. Copyright@ International Science Community Association