International Science Community Association International Research Journal of Biological Sciences 2278-3202 7 10 2018 10 10 An improved protocol for genomic DNA extraction and purification from leaves of Terminalia Arjuna (Roxb.) Wight and Arnis 10 16 EN Nath Susanta 1 Government General Degree College Singur-712 409, West Bengal, India Paul Pritam 2 Bidhannagar College EB-2, Sector-1, Salt Lake, Kolkata-700 064, WB, India Mondal Santanu 3 Bidhannagar College EB-2, Sector-1, Salt Lake, Kolkata-700 064, WB, India Podder Rahul 4 Government General Degree College Singur-712 409, West Bengal, India V.S. Patil 1 Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India H.V. Deshmukh 2 Department of Microbiology Yashavantrao Chavan Institute of Science, Satara-415002, MS, India Bharti Sonu 1 Tropical Forest Research Institute, Jabalpur-483001, Madhya Pradesh, India > Research Paper 2018 7 9 2018 10 10 Several available protocols fail to produce good quality genomic DNA from leaves of Terminalia arjuna that contain the exceptionally high amount of interfering secondary metabolites and polysaccharides. To address this issue, a modified DNA isolation protocol was developed for obtaining quality genomic DNA. Briefly, leaf tissue was macerated in lysis buffer supplemented with PVP, sorbitol and sodium chloride. The macerated material was washed in sorbitol buffer for easy extraction and getting rid of mucilaginous substances present in the sample. Subsequently, the inclusion of high concentrations of B-mercaptoethanol, NaCl, and Triton-x in Cetyltrimethylammonium bromide (CTAB) removed polyphenols and polysaccharides. Phenol: Chloroform: Isoamyl alcohol washings took after by precipitation with NaCl proficiently expelled high protein and polysaccharide impurity. The purity of the isolated genomic DNA was checked spectrophotometrically and confirmed by the running of isolated DNA on 0.8% agarose gel electrophoresis. The yield of pure genomic DNA ranged from 120 to 570ng/µl and purity index (A260/A280) ratio from 1.65 to 2.0. The isolated genomic DNA optimized the RAPD analysis and showed clear amplification in all the germplasm tested. Copyright@ International Science Community Association