International Science Community Association International Research Journal of Biological Sciences 2278-3202 7 10 2018 10 10 Pseudozyma flocculosa Y-1: A potent hydrolytic yeast isolate from the biogas digester effluent 6 9 EN Nath Susanta 1 Government General Degree College Singur-712 409, West Bengal, India Paul Pritam 2 Bidhannagar College EB-2, Sector-1, Salt Lake, Kolkata-700 064, WB, India Mondal Santanu 3 Bidhannagar College EB-2, Sector-1, Salt Lake, Kolkata-700 064, WB, India Podder Rahul 4 Government General Degree College Singur-712 409, West Bengal, India V.S. Patil 1 Department of Microbiology, Lal Bahadur Shastri College of Arts, Science and Commerce, Satara-415002, MS, India H.V. Deshmukh 2 Department of Microbiology Yashavantrao Chavan Institute of Science, Satara-415002, MS, India > Research Paper 2018 7 5 2018 10 10 Vegetable waste serves as source of nuisance in markets. The current unscientific disposal methods lead to environmental pollution. Biomethanation is a promising biological treatment method for vegetable wastes. Biomethanation process converts organic matter into biogas and manure. The different types of microorganisms are involved in production of biogas. Hydrolysis is the first step in biomethanation and different kinds of aerobic and anaerobic microorganisms are involved in the process. The biomethanation experiment was carried out in 5litre biogas digester under ambient temperature conditions. The yeasts were isolated by using Sabourauds agar medium. The yeast isolates were subjected to determine their ability for the production of hydrolytic enzymes. The yeast isolate with maximum hydrolytic potential was identified by morphological, cultural, biochemical characterization and molecular identification by 24 S rRNA. Copyright@ International Science Community Association