International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 8 2017 08 10 MicroRNAs Specific Primer Design using miRNA Design Tool 44 46 EN Shaba A.M. 1 Department of Biological Sciences, Niger State Polytechnic Zungeru, Niger State, Nigeria Oyeleke S.B. 2 Department of Microbiology, Federal University of Technology, Minna, Nigeria Oyewole O.A. 3 Department of Microbiology, Federal University of Technology, Minna, Nigeria Mohammed S.S.D. 4 Department of Microbiology, Kaduna State University, Kaduna State, Nigeria Salahudeen A.O. 5 Department of Microbiology, Federal University of Technology, Minna, Nigeria Ikekwem C.C. 6 Department of Microbiology, Federal University of Technology, Minna, Nigeria Niranjan Nandkumar Patil 1 Department of Microbiology, Patkar-Varde College, S.V. Road, Goregaon, Mumbai, India Shweta Bhise Patel 2 Department of Microbiology, Patkar-Varde College, S.V. Road, Goregaon, Mumbai, India Anup Kumar Sarkar 1 Department of Botany, Dukhulal Nibaran Chandra College, Aurangabad, Murshidabad, Pin-742201, West Bengal, India and Department of Botany, Prasanna Deb Women’s College, Jalpaiguri, Pin-735101, West Bengal, India Manas Dey 2 Jurapani High School, Jurapani, Dhupguri, Jalpaiguri, Pin-735210, West Bengal, India Mallika Mazumder 3 Department of Botany, Raiganj University, Uttar Dinajpur, Pin-733134, West Bengal, India Jalil Ahmad 1 Department of Biological sciences, University of South Asia, Lahore, Pakistan Ayesha Ameen 2 Department of Biological sciences, University of South Asia, Lahore, Pakistan Shahid Raza 3 Department of Biological sciences, University of South Asia, Lahore, Pakistan Victor Singh Ayam 1 Department of Botany, Rajiv Gandhi University, Rono Hills, Itanagar 79111, Arunachal Pradesh, India Pradip Doley 2 Department of Botany, Rajiv Gandhi University, Rono Hills, Itanagar 79111, Arunachal Pradesh, India Ch. B. Singh 3 Institute of Bioresource and Sustainable Development, Takyelpat, Imphal 795001, Manipur, India Maulik Rachh 1 Veer Narmad South Gujarat University, Surat, Gujarat, India Piyush Desai 2 Veer Narmad South Gujarat University, Surat, Gujarat, India > Short Communication 2017 5 27 2017 08 10 MicroRNAs (miRNAs) are tiny (only 18-24 nucleotide long) non-coding RNAs which involved in post-transcriptional regulation of gene expression in multi-cellular organisms by affecting both translation of mRNAs and the stability. There are several different methods used for quantification of miRNAs like Northern Blotting, quantitative RT-PCR, Microarray etc. but, quantitative RT-PCR is used as the standard that is used to validate & confirmation of the results of various methods. The design of primers for miRNA qRT PCR is very much difficult because of short length of miRNA, which size is the very much near to the length of normal PCR primers. The miRNA Design Tool is based on the Universal Probe Library (ULP) probes to design primer(s) for miRNA detection. The tool is a software based and easy method for design of working primers for target specific miRNA for qRT-PCR. The application is available as online service by AstridBio. Copyright@ International Science Community Association