International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 6 2017 06 10 In vitro bioactivity and phytochemical characterization of Nypa fruticans. Wurmb: a mangrove from Kerala, India 41 51 EN Raza Shahid 1 Lahore Garrison University, Lahore Pakistan Shoaib Muhammad Waseem 2 District Head Quarter Hospital (DHQ), Faisalabad, Pakistan Mubeen Hira 3 Lahore Garrison University, Lahore Pakistan Sofia S. 1 Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India Merlee Teresa M.V. 2 Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India Pinkie Cherian 1 Department of Botany, St.Teresa’s College, Ernakulam, Kerala-682011, India Sheela D. 2 Department of Botany, St.Teresa’s College, Ernakulam, Kerala-682011, India Durga K.V. 3 Department of Botany, St.Teresa’s College, Ernakulam, Kerala-682011, India K.V. Durga 1 1Department of Botany, St. Teresa’s College EKM, Kerala, India D. Sheela 2 1Department of Botany, St. Teresa’s College EKM, Kerala, India Nair Neethi C. 3 1Department of Botany, St. Teresa’s College EKM, Kerala, India Nair Neethi C. 1 Department of Botany, St. Teresas College, Ernakulam, Kerala, India Sheela D. 2 Department of Botany, St. Teresas College, Ernakulam, Kerala, India Cherian Pinkie 3 Department of Botany, St. Teresas College, Ernakulam, Kerala, India Viththalanai Khushbu H. 1 Department of Biosciences, Saurashtra University Rajkot 360 005, Gujarat, India Soni Vinod C. 2 Department of Biosciences, Saurashtra University Rajkot 360 005, Gujarat, India M.S. Lovly 1 Department of Botany, St’ Teresa’s College, Ernakulam, Kerala, India M.V. Merlee Teresa 2 Department of Botany, St’ Teresa’s College, Ernakulam, Kerala, India > Research Paper 2017 3 22 2017 06 10 Nypa fruticans Wurmb is an underutilized mangrove plant in India. Mangroves generally contain more secondary metabolites to thrive in a saline environment. The present study focuses on estimation of primary metabolites like carbohydrates, proteins, lipids etc and secondary metabolites like flavonoids, phenols, tannins etc from different parts of Nypa plant such as leaf, stem, male flower, bracts, unripe endosperm and ripe endosperm. Total phenolic content (79.91±1.63 mg/g) and flavonoid contents (99.5±0.936mg/g) were high in the leaves compared to young and mature endosperm. The antioxidant activity using DPPH and SOD scavenging assay also showed very high in leaves. IC50 value of leaves for DPPH scavenging assay was 6.11±1.68 µg/ml and SOD scavenging assay was 11.85±1.16 µg/ml. GC-MS and LC-MS analysis of NFME of leaves identified several bioactive compounds. Brenzcatechin, 4-vinylphenol, 4-vinyl 2-methoxy-phenol, hexadecanoic acid etc were some of the compounds obtained from GC-MS analysis. In vitro study of methanolic extract for anti-diabetic, anti-arthritic, and anti-inflammatory activity were evaluated and % inhibition was high. Percentage of & Copyright@ International Science Community Association 593;-amylase inhibition of the NFME at 500 µg/ml concentration was 69.87% which showed a significant level of anti diabetic activity. Inhibition of haemolysis of erythrocytes at high temperature was 72.94% which indicated the anti inflammatory potential of NFME. Protein denaturation inhibition of methanolic extract was also significant (53.08%) that showed the anti arthritic activity.