International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 6 2017 06 10 Isolation of bioactive compounds by GC-MS and biological potentials of acanthus ilicifolius, L. 7 19 EN Raza Shahid 1 Lahore Garrison University, Lahore Pakistan Shoaib Muhammad Waseem 2 District Head Quarter Hospital (DHQ), Faisalabad, Pakistan Mubeen Hira 3 Lahore Garrison University, Lahore Pakistan Sofia S. 1 Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India Merlee Teresa M.V. 2 Dept. of Botany, St. Teresa’s College, Ernakulam-682011, Kerala, India > Research Paper 2017 4 22 2017 06 10 Mangroves are ecological group of halophytic plant species, which provide a wide range of pharmaceutical and economic products and services. The present work focussed on a study of Acanthus ilicifolius, L.a mangrove plant from Kerala. Phytochemical constituents were determined by UV-Visible spectrophotometer. Free radical scavenging activity was estimated using in vitro methods like DPPH, Nitric Oxide Radical, ABTS and Super Oxide Dismutase while minimum inhibitory concentration of leaf extracts were determined by resazurin based microtiter dilution assay and the characterisation of bioactive components by GC-MS analysis. The methanol extract of A. ilicifolius, L. leaf showed better scavenging activity in SOD method (419 µg/ml) followed by ABTS (450.7 µg/ml), NO (556 µg/ml) and DPPH (562.5 µg/ml). The crude methanolic extract showed remarkable MIC of 0.469 mg/ml against Staphylococcus aureus, 0.521 mg/ml against Klebsiella pneumonia, 0.416 mg/ml against Candida albicans and Pencilliumnotatum. Phenols, flavonoids and tannins had higher correlation with antioxidant activities. The GC-MS of active column fraction revealed that the active principles were a mixture of Lupeol, decanoic acids, cyclolignan glycosides, glycine, cyano colchicine and other therapeutically active compounds. It could be concluded that A .ilicifolius, L. contains compounds with various biological activities and so recommended as a plant of phyto pharmaceutical importance. Copyright@ International Science Community Association