International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 4 2017 04 10 Statistical optimization of cellulase production in Pseudomonas spp and its application in bioethanol production 21 26 EN M. Sreenivas 1 Department of Zoology, Kakatiya University, Warangal-506009, Telangana, India G. Shamitha 2 Department of Zoology, Kakatiya University, Warangal-506009, Telangana, India Nwanyanwu C.E. 1 Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria Adieze I.E. 2 Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria Nweke C.O. 3 Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria Nzeh B.C. 4 Department of Microbiology, Federal University of Technology, P.M.B. 1526, Owerri, Nigeria B. Sasirekha 1 Department of Microbiology, Acharya Bangalore B School, Off Magadi road, Bangalore, Karnataka, India B.J. Vishal 2 Department of Biotechnology, Acharya Bangalore B School, Off Magadi road, Bangalore, Kaarnataka, India > Research Paper 2017 2 9 2017 04 10 Cellulolytic microorganism converts cellulose into monomeric units which can be used for production of valuable products. This study demonstrates the production of cellulase by Pseudomonas spp VC14. Strain Pseudomonas spp VC14 cellulase activity was increased by optimization of physicochemical parameters by Plackett-Burman statistical design. Optimization showed NH4Cl and FeSO4 as significant components influencing cellulose production. On optimization the production of cellulase increased 5.41 fold (1.84 U/ml) within 24 hrs compared to unoptimized medium. Simultaneous saccharification and fermentation of reducing sugars with Saccharomyces cerevisae showed 5.1% bioethanol production. Thus, the results of the present work clearly revealed that cellulose from strain Pseudomonas spp VC14 can efficiently be used for bioethanol production. Copyright@ International Science Community Association