International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 3 2017 03 10 Fabrication and operation of a novel mediator and membrane-less microbial fuel cell 8 13 EN Senthilkumar S.R. 1 Department of Botany, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India Sivakumar T. 2 Department of Botany, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India Arulmozhi K.T. 3 Physics Wing (DDE), Annamalai University, Annamalainagar 608 002, Tamil Nadu, India Mythili N. 4 Department of Physics, Mohamed Sathak Engineering College, Kilakarai-623806, Ramnad, India S. Geetha 1 Department of Microbiology, The Standard Fireworks Rajaratnam College for Women Sivakasi, Tamilnadu, India Ranjani S. Subha 2 Department of Microbiology, The Standard Fireworks Rajaratnam College for Women Sivakasi, Tamilnadu, India > Research Paper 2017 1 21 2017 03 10 This present study deals with the Microbial Fuel Cells (MFC) using waste water and waste organic matter in its organic content to generate electricity using microorganisms. Microorganisms were able to utilize the carbon source in the substrate for generation of bioelectricity. This study is concentrated on the comparison of electricity generation by two different organic substrates like whey, rotten tomato juice, and electricity generation with Saccharomyces cerevisiae and Escherichia coli and also the comparative study on two different combinations of electrodes Carbon-Copper and Graphite-Copper. Electrodes play an important role in microbial fuel cells. In Carbon-Copper electrode whey water produced maximum voltage of 934mV at 2nd day of incubation. The result as electricity output was recorded as open circuit voltage (OCV) by Digital multimeter. Copyright@ International Science Community Association