International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 6 1 2017 01 10 Synthesize a transfer vector containing the gene encoding ADP-glucose pyrophosphorylase enzyme to improve the starch content in Manihot esculenta Crantz 25 33 EN Dawane Vinars 1 School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar – 382030, Gandhinagar, Gujarat, India Fulekar M.H. 2 School of Environment and Sustainable Development, Central University of Gujarat, Gandhinagar – 382030, Gandhinagar, Gujarat, India Dieudonne Mutangana 1 University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda Innocent Uwintwali 2 University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda Eugѐne Ndisanze M. 3 University of Rwanda, College of Science and Technology, Department of Biology, Avenue de l’Armée, Po. Box 3900, Kigali-Rwanda Mahurpawar Manju 1 Dept. of Zoology, Govt P.G. College, Parasia (Chhindwara) M.P., India Khatoon Akhtari 1 P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India Mohapatra Ashirbad 2 Sri Jayadev College of Education and Technology, Naharkanta, Bhubaneswar-752101, Odisha, India Satapathy Kunja Bihari 3 P.G. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, India Lan Do Hai 1 Faculty of Biology - Chemistry, Tay Bac University, Quyet Tam, District - Sơn La City, Vietnam Son Le Van 2 Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam Binh Le Tran 3 Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Ha Noi, Vietnam > Research Paper 2016 12 18 2017 01 10 Starch is polysaccharide which plays important role in the metabolism of living organisms. In addition, starch is raw materials in the food and materials industry. Increasing the starch content of crops based on improving the activity of some genes encoding enzymes plays an important role in the process of biosynthesis and accumulation of starch, which is one of the research are of interest. Like in bacteria, the process of starch biosynthesis in plants occurs by using ADP-Glc as raw materials to made long link chain & Copyright@ International Science Community Association 945;-1.4-glucoside. ADP-Glcpyrophosphorylase (AGPase) has an important role in the biosynthesis of ADP-Glc molecule and therefore AGPase is a critical enzyme of glycogen synthesis in bacteria and starch in plants. In this study the gene encoding for AGPase enzyme (symbol Lan1_opt) size 1.5 kb originating from the glgC gene coding for the enzyme AGPase of E. coli has been optimised. This gene had G336 mutation replacing aspartic acid by glycin in order to decrease the affinity of the inhibitor. It has been combined with gene transfer plant vector and was transformed into tobacco plants operated under the control of a 35S promoter. The integration of the Lan1_opt gene into plant genome was confirmed by PCR technique showing the expression of AGPase proteins in the genomic tobacco plants. Moreover AGPase enzyme activity in leaves of this genomic tobacco plants was higher than 21%-43% compared with the AGPase enzyme activity in leaves of tobacco plants control. Our data provided the gene encoding AGPase (Lan1_opt) was an effective strategy to strength the process of starch biosynthesis in plants.