International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 6 2016 06 10 Anticancer and Cytotoxic Potential of Ethanolic extract of Tribulus terrestris on Hela cell lines 37 42 EN AI-Jawhari Ihsan Flayyih Hasan 1 Department of Environment and Pollution, Marshes Research Centre, Thi-Qar University, Iraq M. Gutierrez Jr. Pedro 1 Department of Biology, College of Arts and Sciences, Cebu Normal University, Cebu City, Philippines R.B. Borse 1 Nagnath Art, Commerce and Science College Aundha (Nagnath) Dist. Hingoli, MS, India Dilla Jose* 1 PG and Research Department of Zoology, Sir Theagaraya College, Chennai, Tamil Nadu, India P. Senthilkumaar 2 PG and Research Department of Zoology, Sir Theagaraya College, Chennai, Tamil Nadu, India R* Kujur 1 School of Life Sciences, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India A Ekka 2 School of Life Sciences, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India J.* Dhanalakshmi 1 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India Selvi V. Senthamarai 2 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India S. Selvi 3 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India > Research Paper 2016 4 29 2016 06 10 Tribulus terrestris Linn., belongs to family Zygophyllaceae. It is popularly known as puncture vine plant. It is a perennial creeping herb commonly distributed worldwide. The objective of the work is to find out the effectiveness of anticancer potential in ethanolic extract in fruits of T. terrestris. In the phytochemical analysis the ethanolic extract in fruits of T. terrestris showed the presence of Coumarin, Phenol, Phytosterols, Protein, Saponin and Tanins as positive results. The cytotoxic effect was determined by MTT assay and Trypan Blue assay. The cytotoxicity reactivity is moderate in 10µl and 5.0µl and mild in 2.5µl concentration in ethanolic extract in fruits of T. terrestris. The ethanolic extract of T. terrestris given mild to moderate cytotoxicity on L929 mouse fibroblast cells. The results suggested that the existence of bioactive compounds in the ethanolic extract in fruits of T. terrestris revealed a strongest anticancer activity against cervical cancer cell line (HeLa). Copyright@ International Science Community Association