International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 6 2016 06 10 Purification of toxin protein from E. coli isolate pe88 57 59 EN AI-Jawhari Ihsan Flayyih Hasan 1 Department of Environment and Pollution, Marshes Research Centre, Thi-Qar University, Iraq M. Gutierrez Jr. Pedro 1 Department of Biology, College of Arts and Sciences, Cebu Normal University, Cebu City, Philippines R.B. Borse 1 Nagnath Art, Commerce and Science College Aundha (Nagnath) Dist. Hingoli, MS, India Dilla Jose* 1 PG and Research Department of Zoology, Sir Theagaraya College, Chennai, Tamil Nadu, India P. Senthilkumaar 2 PG and Research Department of Zoology, Sir Theagaraya College, Chennai, Tamil Nadu, India R* Kujur 1 School of Life Sciences, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India A Ekka 2 School of Life Sciences, Pt. Ravishankar Shukla University, Raipur, Chhattisgarh, 492010, India J.* Dhanalakshmi 1 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India Selvi V. Senthamarai 2 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India S. Selvi 3 Department of Biochemistry, Bharathidasan College of Arts and Science, Erode, Tamil Nadu, India K. Chandra Prabha* 1 Department of Biochemistry, Navarasam Arts & Science College, Arachalur, Erode (DT)-638101, TN, India S. Selvi 2 Department of Biochemistry, Bharathidasan College of Arts & Science, Ellispettai, Erode (DT)-638116, TN, India Sharif Khan 1 Mewar University, Chittorgadh Rajasthan, India V.S.* Nagpurne 1 Department of Botany Maharashtra Udayagiri Mahavidyalaya, Udgir, Dist. Latur MS, India J.M. Patwari 2 Department of Environmental Science, Maharashtra Udayagiri Mahavidyalaya, Udgir, Dist. Latur MS, India Mary Conice* 1 Dept. of Microbiology, Centre for P.G. Studies, 18/3, 9th Cross, 3rd Block, Jayanagar, Jain University, Bangalore-11, India M.S. Usha 2 Dept. of Microbiology, Centre for P.G. Studies, 18/3, 9th Cross, 3rd Block, Jayanagar, Jain University, Bangalore-11, India > Short Communication 2016 3 28 2016 06 10 Escherichia coli isolate pe88, which showed positive to Stx2c toxin by PCR analysis was selected for the present study. The protein from E. coli culture was subjected to salting out by ammonium sulphate precipitation till the saturation followed by dialysis using 1% sucrose solution for overnight. Further purification of protein from the isolate was carried out by ion-exchange chromatography. Column was developed using DEAE Cellulose for anion and Sephrose for cation exchanger. Elution buffer was used as mobile phase. Procedures were performed at room temperature. Elutions collected every five minutes were subjected to protein estimation by Lowry’s method using Bovine Serum Albumin as standard. The elution of protein from the isolate pe88 was subjected to SDS-PAGE using standarded molecular weight marker. The sample showed a single band close to 74 kDa after purification by column chromatography. Copyright@ International Science Community Association