International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 12 2016 12 10 Microclonal Propagation of Grapevine (Vitis vinifera L.) cv. Al-Bayadi. Grown in Taif, KSA 24 27 EN N.M. Aka 1 Oceanological Research Center (Abidjan, Côte d’Ivoire), BPV 18, Abidjan R.N. Etilé 2 Hydrobiology Laboratory, UFR Biosciences, University Félix HOUPHOUËT-BOIGNY (Côte d’Ivoire), 22 BP 582 Abidjan, Côte d’Ivoire F.K. Konan 3 Department of Environment, University Jean Lorougnon Guédé, BP 150 Daloa, Côte d’Ivoire Y.K. Bony 4 Department of Environment, University Jean Lorougnon Guédé, BP 150 Daloa, Côte d’Ivoire Melaku Yadessa 1 Program of Chemistry, Adama Science and Technology University, Adama, Ethiopia Duguma Tolessa 2 Program of Chemistry, Adama Science and Technology University, Adama, Ethiopia Khamtilin Kharshiing 1 Department of Zoology, Lady Keane College, Shillong-793 001, India Gupta B.B.P. 2 Environmental Endocrinology Laboratory, Department of Zoology, North-Eastern Hill University, Shillong – 793 022, India S. Dessoky El dessoky 1 Biotechnology and Genetic Engineering Research Unit, Scientific Research Center, Taif University, Taif, Al-Haweiah, P.O. Box 888, Zip code 21974, Taif, KSA O. Attia Attia 2 Agricultural Genetic Engineering Research Institute, Agricultural Research Center, P.O. Box, 12619, Giza, Egypt > Research Paper 2016 11 9 2016 12 10 A protocol for rapid multiplication of Grapevine (Vitis vinifera L.) cv. Al-Bayadi. Grown in Taif, Saudi Arabia was developed using shoot tips and internodes segments explants excised from mature plants in filed. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of BAP (6-Benzylaminopurine) (0.0, 0.5, 1.0, 1.5 and 2 mg/l) to investigate in vitro stages shoot initiation, multiplication and elongation. Effect of different concentrations of IBA (Indole-3-butyric acid) (0.0, 0.1, 1.0 and 2 mg/l) and 0.1 mg/l of NAA (Naphthalene acetic acid) on root formation of shoots were studied. The highest percentage of shoot initiation (90%), maximum average number of multiplied shoots (3.7) and highest average number of elongated shoots (3.6) were observed on MS medium supplemented with 2 mg/l BAP. For rooting, highest percentage (100%) of rooted shoots was obtained on MS medium supplemented with 2 mg/l IBA+0.1 mg/l NAA. Plantlets with 4 to 5 roots of 3 to 5 cm length were transferred to pots containing sterile peat moss for acclimatization in greenhouse. Copyright@ International Science Community Association