International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 10 2016 10 10 Extraction and Characterization of L-asparaginase from Spinacea oleraceae 45 50 EN SABER Seyyed Naeim 1 Cukurova University, Department of Animal Science, Adana, Turkey A. Mahadeva 1 Residential Coaching Academy, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae Barely Road, Lucknow – 226 025, India S.L. Sevidzem 1 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon A. Mamoudou 2 University of Ngaoundéré, School of Veterinary Medicine and Sciences, Department of Parasitology and Parasitological Diseases, Ngaoundéré, Cameroon G.L. Acapovi-Yao 3 Université Félix Houphouët-Boigny, UFR Biosciences 22, BP 582 Abidjan 22, Côte d’Ivoire M. Achiri 4 Mission Spéciale d\'Eradication des Glossines, Nord et L’Extreme Nord, Cameroun T. Tchuinkam 5 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon K.C.R. Zinga 6 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon J.F. Mavoungou 7 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon and Université des Sciences et Techniques de Masuku, BP : 941 Franceville, Gabon Kumar Mukesh 1 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Thapliyal Manisha 2 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Singh Ajeet 3 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Chauhan Rekha Rani 1 Department of Botany, KGK (PG) College, Moradabad-244001, Uttar Pradesh, India Singh Alka 2 Plant Physiology Lab., Department of Botany, Hindu College Moradabad-244001, Uttar Pradesh, India Singh Pramod Kumar 3 Plant Physiology Lab., Department of Botany, Hindu College Moradabad-244001, Uttar Pradesh, India N. Bajpai 1 Govt Degree College, Kaushambi, Uttar Pradesh, India Marcel Andzouana 1 Department of Chemistry, Faculty of Sciences and Technics, Marien NGOUABI University, Brazzaville, Congo Hubert Makomo 2 Department of Chemistry, Faculty of Sciences and Technics, Marien NGOUABI University, Brazzaville, Congo Khabade Sarina P. 1 Department of PG studies and Research in Biotechnology, Government Science College, Bangalore-01, India Patel Mahir S. 2 Department of PG studies and Research in Biotechnology, Government Science College, Bangalore-01, India R. Sowjanya 3 Department of PG studies and Research in Biotechnology, Government Science College, Bangalore-01, India > Research Paper 2016 9 16 2016 10 10 L-asparaginase (E.C. 3.5.1.1) is an enzyme that catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia. It is identified as an effective antitumor agent in human clinical trials and is now recognized as one of the important component of antitumor therapy. The purpose of the present study was to screen for the production of L-asparaginase enzyme in Spinacea oleracea. The presence of the enzyme was confirmed by the formation of ammonia which was detected using UV-Visible Spectrophotometer at 460nm. The enzyme activity was found to be 1.9IU and specific activity was 6.8 µmoles/mg/min. The enzyme kinetics revealed that the optimum pH of the enzyme L-asparaginase was 8.0, optimum temperature was found to be 370C and optimum time was 15 minutes. The Km and V-max values of Spinacia oleracea was found to be 5 and 2.5 respectively by means of the double-reciprocal Lineweaver-Burk plot. Copyright@ International Science Community Association