International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 10 2016 10 10 DNA-Based Characterization of Flesh Flies (Diptera: Sarcophagidae) 35 39 EN SABER Seyyed Naeim 1 Cukurova University, Department of Animal Science, Adana, Turkey A. Mahadeva 1 Residential Coaching Academy, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae Barely Road, Lucknow – 226 025, India S.L. Sevidzem 1 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon A. Mamoudou 2 University of Ngaoundéré, School of Veterinary Medicine and Sciences, Department of Parasitology and Parasitological Diseases, Ngaoundéré, Cameroon G.L. Acapovi-Yao 3 Université Félix Houphouët-Boigny, UFR Biosciences 22, BP 582 Abidjan 22, Côte d’Ivoire M. Achiri 4 Mission Spéciale d\'Eradication des Glossines, Nord et L’Extreme Nord, Cameroun T. Tchuinkam 5 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon K.C.R. Zinga 6 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon J.F. Mavoungou 7 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon and Université des Sciences et Techniques de Masuku, BP : 941 Franceville, Gabon Kumar Mukesh 1 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Thapliyal Manisha 2 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Singh Ajeet 3 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Chauhan Rekha Rani 1 Department of Botany, KGK (PG) College, Moradabad-244001, Uttar Pradesh, India Singh Alka 2 Plant Physiology Lab., Department of Botany, Hindu College Moradabad-244001, Uttar Pradesh, India Singh Pramod Kumar 3 Plant Physiology Lab., Department of Botany, Hindu College Moradabad-244001, Uttar Pradesh, India N. Bajpai 1 Govt Degree College, Kaushambi, Uttar Pradesh, India > Research Paper 2016 9 9 2016 10 10 The members belonging to the family Sarcophagidae includes species of medically, veterinary and forensic importance. Many studies have been carried out on the cytogenetics and biochemical genetics of these flies; however, very little work has been carried out using DNA based methods for their molecular characterization. Therefore, in the present study amplification of Cytochrome b gene was performed among five sarcophagids viz., Sarcophaga knabi, S. albiceps, S. dux, S. argyrostoma and S. ruficornis with a view to unravel the genetic relationship among these Indian flesh flies. DNA sequence was analysed using MEGA 4 software. Phylogenetic analysis was also performed which is in congruence with the results found earlier by using COI gene. The result shows the reliability of Cyt b gene as a diagnostic marker for the genetic study of these flesh flies. Copyright@ International Science Community Association