International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 5 10 2016 10 10 Isolation and Identification of α- Amylase activity Inhibiting compounds from Bryophyllum Pinnatum 21 27 EN SABER Seyyed Naeim 1 Cukurova University, Department of Animal Science, Adana, Turkey A. Mahadeva 1 Residential Coaching Academy, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Rae Barely Road, Lucknow – 226 025, India S.L. Sevidzem 1 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon A. Mamoudou 2 University of Ngaoundéré, School of Veterinary Medicine and Sciences, Department of Parasitology and Parasitological Diseases, Ngaoundéré, Cameroon G.L. Acapovi-Yao 3 Université Félix Houphouët-Boigny, UFR Biosciences 22, BP 582 Abidjan 22, Côte d’Ivoire M. Achiri 4 Mission Spéciale d\'Eradication des Glossines, Nord et L’Extreme Nord, Cameroun T. Tchuinkam 5 Vector-Borne Infectious Disease Unit, Laboratory of Applied Biology and Ecology (VBID-LABEA), Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon K.C.R. Zinga 6 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon J.F. Mavoungou 7 Laboratoire d’Ecologie Vectorielle (LEV), Département de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET), BP: 13354, Libreville, Gabon and Université des Sciences et Techniques de Masuku, BP : 941 Franceville, Gabon Kumar Mukesh 1 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Thapliyal Manisha 2 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India Singh Ajeet 3 Department of Biotechnology, G.B. Pant Engineering College, Pauri Garhwal, Uttarakhand, India > Research Paper 2016 7 20 2016 10 10 Bryophyllum pinnatum (B. Pinnatum) is an herb growing widely and utilized in folkloric medication in tropical Africa, tropical America, India, China, and Australia. Aqueous extract of B. pinnatum leaf was tested for alpha amylase inhibition properties which showed significant inhibitory activity against alpha amylase. B. pinnatum found to have maximum alpha amylase inhibitory activity at a concentration of 100µg/ml. A compound Digitoxin showed maximum binding energy with alpha amylase comparative to drug Metformin, Metformin at concentration 100 µg/ml showed 67.34±0.002% inhibitory effects on the alpha-amylase activity with an IC50 value 3.25µg/ml. The alpha-amylase inhibitory activity of 79.41±0.002% with an IC50 value 2.54µg/ml was shown by aqueous leaf extract of B. pinnatum at a concentration 100 µg/ml. The plant extract showed potential alpha-amylase inhibitory activity in a dose dependent manner. Copyright@ International Science Community Association