International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 2 2 2013 February 10 Bioautography guided Screening of Antimicrobial Compounds Produced by Microbispora V2 65 38 and Bankar A.V. EN Farzaneh Hosseini 1 Faculty of Bioscience, Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, IRAN Adlgostar Afsoon 2 Faculty of Bioscience, Department of Biology, Islamic Azad University, North Tehran Branch, Tehran, IRAN Fariba Sharifnia 3 Panchal HetalK. 1 Dolat Usha Institute of Applied Sciences, Valsad; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA Trivedi RatnaA. 2 Shree Ram Krishna Institute of Comp. Edu. and Applied Sciences; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA Desai PratibhaB. 3 Venkata Saibabu 1 Dept. of Biochemistry, Center for Post Graduate Studies, Jain University, Jayanagar 3rd Block, Bangalore-560011, Karnataka, INDIA Francois NiyongaboNiyonzima 2 More SunilS. 3 Choubey K. 1 Deptt. of Zoology, G.V.Y.T. College, Durg, CG, INDIA Qureshi Y. 2 Deptt. of Zoology, Govt. College, Khertha, Distt. Balod, CG, INDIA Kulkarni D.A. 1 Department of Zoology, S.M.D.M. Mahavidyalyaya, Kallamb, Dist. Osmanabad, MS, INDIA Surwase S.S. 2 Department of Zoology, Rajaram College, Kolhapur, MS, INDIA NoorAzlina Ibrahim 1 Faculty of Agro Based Industry, Universiti Malaysia Kelantan (Jeli Campus), Locked Bag No. 100, 17600 Jeli, Kelantan, MALAYSIA Yusoff Norazila 2 Baradaran Samaneh 1 Department of Medical Radiation Engineering, Amirkabir University of Technology, Tehran, IRAN Setayeshi Saeed 2 Faculty of Engineering, Science and Research Branch, Islamic Azad University, Tehran, IRAN Niaz Maleknasr 3 Radiation Application School, Nuclear Sciences and Technology Research Institute, Tehran, IRAN Kardan MohammadR. 4 Singh Ajai 1 1Department of Orthopaedics, King George's Medical University, Lucknow-226003, UP, INDIA Sabir Ali 2 Department of Biochemistry, King George's Medical University, Lucknow-226003, UP, INDIA Mahdi A.A. 3 Srivastava R.N. 4 NayakPavithra P. 1 Centre for Applied Genetics, Jnanabharathi Campus, Bangalore University, Bangalore - 560 056, Karnataka, INDIA Reddy ShankarM. 2 Department of Zoology, Jnanabharathi Campus, Bangalore University, Bangalore - 560 056, Karnataka, INDIA Jayaprakash 3 Alfarra H.Y. 1 Dept. of Biotechnology, Kulleyyah (Faculty) of Science, International Islamic University Malaysia, Kuantan, Pahang, MALAYSIA Hasali N.H.M. 2 Omar M.N. 3 Attarzadeh RezaSeyed 1 Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, IRAN Abbasian Sadegh 2 Faculty of Physical Education and Sport Sciences, University of Tehran, IRAN Azimkhani Amin 3 Imamreza university of Mashhad, Mashhad, IRAN Onvani Vahid 4 Faculty of Physical Education and Sport Sciences, Azad University of Mashhad, IRAN Joghataie Mohsen 5 B. NakadeDhanraj 1 Govt. of Maharashtra Rajaram College, Kolhapur-416004, Maharashtra, INDIA Patil N.N. 1 Department of Microbiology, PDEA’s Annasaheb Magar Mahavidyalaya, Hadapsar, Pune- 411028, MS, INDIA Waghmode M.S. 2 Department of Microbiology, University of Pune, Ganashkhind, Pune-411007, MS, INDIA Gaikwad P.S. 3 Gajbhai M.H. 4 > Research Paper 2012 12 14 2013 February 10 The aim of this study was to investigate the antimicrobial activity of MicrobisporaV2. The genusMicrobispora is known to produce antimicrobial compounds like phenazines and others. The isolate Microbispora V2 can be exploited to mine out its capabilities of producing antimicrobial compounds utilizing cost effective production media. The screening is defined as the first step, which is applied to a sample, in order to establish the presence or absence of antimicrobial compounds. Bioautography belongs to microbiological screening methods commonly used for the detection of antimicrobial activity of a compound under study. Bioautography is a technique that combines thin Layer chromatography with bioassay in situ. It is one of the simplest and cheapest methods for detecting antimicrobial compounds in partially purified extracts because the method is easy to run, reproducible and requires less equipments. The fermentation process can be made cost effective by utilizing renewable energy sources like oil cake as sources of macronutrients of fermentation media. Therefore various oil cakes were chosen as nutrient source of fermentation media. Hence bioautography was used as a tool to screen out the production of antimicrobial compounds by Microbispora V2 employing oil cake fermentation media. Copyright@ International Science Community Association