International Science Community Association International Research Journal of Biological Sciences 2278-3202 13 4 2024 11 10 Utilization of Humulene for the Silkworm Cocoon and Silk yield 37 47 EN Chaturvedi Prashant 1 Department of Biological Science, Rani Durgavati University, Jabalpur, MP, India Singh Divya 2 Department of Biological Science, Rani Durgavati University, Jabalpur, MP, India Pathak Renu 3 Department of Biological Science, Rani Durgavati University, Jabalpur, MP, India Beohar Purnima 4 Department of Biological Science, Rani Durgavati University, Jabalpur, MP, India ORTESE 1 Department of Microbiology, Joseph Sarwuan Tarka University, Makurdi, Benue State, Nigeria Aondover Raymond 2 Lin Su Su Mar 1 Department of Food Science, Mae Fah Luang University, Thailand and Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar and Department of Advanced Science and Technology, Nay Pyi Taw, Myanmar Myint Myo 2 Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar Chukeatirote Ekachai 3 Department of Food Science, Mae Fah Luang University, Thailand I. Mady Goma Dirat 1 Laboratoire de Recherche en Biologie et Ecologie Animales, Ecole Normale Supérieure, BP 69, Université Marien NGOUABI, Brazzaville, Congo and Faculté des Sciences Appliquées, Université DENIS SASSOU-N’GUESSO, udsn.cg Kintélé, Congo L.P. Boukama 2 Laboratoire de Recherche en Biologie et Ecologie Animales, Ecole Normale Supérieure, BP 69, Université Marien NGOUABI, Brazzaville, Congo A. Tsoumou 3 Laboratoire de Recherche en Biologie et Ecologie Animales, Ecole Normale Supérieure, BP 69, Université Marien NGOUABI, Brazzaville, Congo M. Mikia 4 Laboratoire de Recherche en Biologie et Ecologie Animales, Ecole Normale Supérieure, BP 69, Université Marien NGOUABI, Brazzaville, Congo H. Banga Mboko 5 Ecole Nationale Supérieure d’Agronomie et de Foresterie, BP 69, Université Marien NGOUABI, Brazzaville, Congo Macwan Carol P. 1 Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat, India Upadhyay Nandini P. 2 Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat, India Soni Tejal G. 3 Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat, India Suhagia B.N. 4 Faculty of Pharmacy, Dharmsinh Desai University, Nadiad, Gujarat, India Madgunki Mayuri Ramesh 1 Department of Botany, Shri Shivaji Mahavidyalaya, Barshi Shivaji Nagar Barshi Tal-Barshi, Disrict-Solapur -413401, India Khyade Vitthalrao B. 2 Sharadabai Pawar Mahila Arts, Commerce and Science College, Sharadanagar Tal. Baramati, District-Pune 413115, India > 2024 8 19 2024 11 10 Humulene sesquiterpene compound with single ring and three units of isoprene. The present attempt was aimed to utilize humulene through acetone for applications (topical) on the second day to the fifth larval stage of double hybrid race of silkworm, Bombyx mori (L). The application humulene through acetone was resulted into yield of qualitative and quantitative silk cocoons and silk fibers. The entire cocoon weight (without floss), weight of shell of silk cocoon, weight of pupae. There was significant improvement in the silk shell percentage (mathematical operation of division of shell weight by the weight of whole cocoon. Resulted quotient multiplied by hundred)and scale of denier of silk through the utilization of spray of humulene solution to the fifth larval stage of double hybrid race of silkworm, Bombyx mori (L). The readings: 2.967** (±0.879); 0.843** (±0.137); 2.124 and 28.412*** respectively belong to weight of entire cocoon, weight of silk shell, weigh of pupae and the silk shell percentage (ratio of shell to the entire cocoon). The readings: 1489.63* (±229.53); 0.831** (±0.118) and 5.020*** respectively belong to silk fiber length (meter), silk fiber weight (gram) and the scale of denier. As a terpene compound, humulene exhibits probable activity analogous with natural Juvenile Hormone (JH) and may deserve applicable aspects of its utilization as efficient factor for growth of insects like silkworm. The schedule of spraying humulene through acetone should be introduced in the rearing of larval stages of silkworm for yield of qualitative and quantitative silk cocoons and silk fibers. Copyright@ International Science Community Association