International Science Community Association International Research Journal of Biological Sciences 2278-3202 13 2 2024 05 10 Phytochemical and in vitro bioactivity of Entada africana Guill & Perott (Fabaceae) stem bark methanol extracts on Schistosoma haematobium cercaria 21 28 EN Srivastav Aditi 1 Department of Biotechnology, Rama University, Kanpur, UP, India Pandey Chitranshu 2 R & D Division, MRD Life sciences Pvt. Ltd. Lucknow, UP, India Sharma Pallavi 3 R & D Division, Somics Life sciences Pvt. Ltd. Bareilly, UP, India D.V. Hande 1 Dept. of Plant Biology and Biotechnology, University of Benin, Benin City, Nigeria S.A. Shelke 2 Department of Plant Science and Biotechnology, Federal University of Lafia, PMB 146, Lafia, Nigeria Mahapatra Bingshati Singha 1 Department of Botany, Bankura University, Bankura, West Bengal, India Maji Samir Kumar 2 Department of Chemistry, Ramananda College, Bishnupur, Bankura, West Bengal, India Mallick Shyamal Kanti 3 Department of Botany, Ramananda College, Bishnupur, Bankura, West Bengal, India Daben 1 Department of Science Laboratory Technology, Faculty of Natural Sciences, University of Jos, Jos, Plateau State, Nigeria Rindum Moses 2 Department of Zoology, Faculty of Natural Sciences, University of Jos, Jos, Plateau State, Nigeria Mwansat 3 Samuel Georgina 4 > Research Paper 2024 1 26 2024 05 10 Schistosomiasis, has attained socioeconomic and public health significance, especially in sub-Saharan Africa; ranking second after malaria. Potential resistance to niclosamide, resulted in search for alternative control measures that is cheap and accessible to most rural communities. This study was therefore aimed at screening the bioactive metabolites of Entada africana (E. africana), and larvicidal effects of the stem bark methanol extracts against Bulinus globosus (B. globosus) cercaria. Soxhlet extraction of the crude powder was done using methanol solvent. Qualitative phytochemical screening was carried out using standard procedures. Cercariae were challenged with different concentrations. Dechlorinated water with cercaria was used as control. Rate of mortality, LC50 values and Kaplan-Maier survivorship curve at 60 minutes of post exposure (PE) was assessed. Data were analyzed using GraphPad® Prism version 8.4. Screening revealed the presence of carbohydrates, tannins, saponins, and alkaloids among others. Saponins and carbohydrate was high in the stem bark and leaf extract respectively. At p ≤ 0.05, there was significant difference in rate of mortality; LSD: 11.52, % interaction = 16.00, 95% CI (-2.65 to 9.06). At 50 minutes of PE, all parasites were dead. The LC50, valued at 46.00±6.93, and 100% mortality across all concentrations was at 90mg/l in 10 minutes of PE. Mortality was concentration and time dependent. Control showed motility throughout 60 minutes of PE. The in vitro evaluation of E. africana stem bark methanol extract was potent against the infective larval stage of S. haematobium, and showed high potential as an anticercarial agent. Copyright@ International Science Community Association