International Science Community Association International Research Journal of Biological Sciences 2278-3202 11 4 2022 11 10 Prebiotic potential and characterization of microbes found in the guts of Wistar rats fed with feeds supplemented with some Macro-fungi from Oyo state, Southwestern Nigeria 1 6 EN Omeonu Francis Chukwumma 1 Mycology & Applied Microbiology Unit, Department of Botany, University of Ibadan, Nigeria Salami Adeola Temitope 2 Gastrointestinal Secretion and Inflammatory Research Unit, Department of Physiology, University of Ibadan, Ibadan, Nigeria Azuh Victor Okechukwu 3 Genetics Unit, Department of Botany, University of Ibadan, Nigeria Laba S.A. 4 Department of Microbiology, University of Ilorin, Ilorin Kwara State, Nigeria > Research Paper 2021 11 26 2022 11 10 The new phenomenon in food technology is the use of prebiotics for therapeutic purposes to control the normal body flora of gastrointestinal tracts. The prebiotic potential and characterization of microbes found in the guts of Wistar rats fed with feeds supplemented with some selected macro-fungi diet was evaluated on male Wistar rats (Rattus norvegicus). Our result showed that the macro-fungi supplemented diets exhibited prebiotic activities while thebacteria isolated and characterized from the stomach of Wistar rat include Lactobacillus casei, Pediococusacidilactici, Lactobacillus fermentum, Enterococcus faecalis, Streptococcus pneumonia, Lactobacillus acidophilus, Leuconostoc lactis, Campylobacter jejuni, Vibroalbensis, Salmonella enterica, Shigella dysenteraie, and Escherichia coli. From the results, it is suggestive that the higher fungi enriched feedsis prebiotic as they enhanced the pro-biotic micro-flora over the pathogenic microbes. Copyright@ International Science Community Association