International Science Community Association International Research Journal of Biological Sciences 2278-3202 10 4 2021 12 10 Decolourization of bromophenol blue and bromocresol green by laccase enzyme from Brassica oleraceae 24 28 EN Samuel Onuorah 1 Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria Dominic Ndumdi 2 Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria P. Sunil Kumar 1 Department of Zoology, Sree kerala Varma College, Thrissur-680001, India A. Sneha 2 Department of Zoology, Sree kerala Varma College, Thrissur-680001, India Parmar B.M. 1 Central Laboratory, Dudheswar waterworks, Ahmedabad Municipal Corporation, Gujarat, India Revathi S. 1 Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India Taj Mohsin 2 Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India Shalini N.S. 3 Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India Khabade Sarina P. 4 Department of biotechnology, Government Science College (Autonomous), Bangalore, Karnataka, India > Research Paper 2021 5 28 2021 12 10 Industrialization has led to a serious threat to water bodies and causing harmful impacts on living organisms. Industries discharge untreated waste water directly into the environment causing environmental pollution. Biotechnologists across the world are trying to develop innovative processes to check global pollution. However, the quantity of various pollutants discharged in the water bodies is increasing tremendously. One of the harmful pollutants is the synthetic dyes from textile industries. They are toxic and carcinogenic and are hazardous to human health. The present Research work focused on solving this problem cost-effectively to some extent. Laccase (E.C.1.10.3.2) is a significant and ubiquitous enzyme which plays avital role in degradation of synthetic dyes. In the current study, laccase enzyme from Brassica oleraceae has been isolated. The substrate used for laccase wasguaiacol. The enzyme activity was found to be 0.83U/ml. The kinetic parameters were studied and optimum conditions recorded were as follows: pH: 4, temperature: 100°C, incubation time: 30 minutes. The crude laccase enzyme was subjected to purification steps that involved ammonium sulphate salt precipitation followed by dialysis and anion-exchange chromatography using DEAE cellulose. The decolorization of synthetic dyes by laccase was seen after incubation of 72hrs. Complete degradation was accomplished in eight days. Copyright@ International Science Community Association