International Science Community Association International Research Journal of Biological Sciences 2278-3202 10 4 2021 12 10 Effect of seasons on the bacteriological quality of Iyifeyi stream in Ugwobi Abbi, Enugu State, Nigeria 1 11 EN Samuel Onuorah 1 Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria Dominic Ndumdi 2 Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria > Research Paper 2020 11 30 2021 12 10 Good quality water is essential to human metabolic activities. The effect of seasons on the bacteriological quality of Iyifeyi stream in Ugwobi Abbi in Uzo-Uwani Local Government Area of Enugu State, Nigeria was studied using the spread plate and multiple tubes methods. Total bacterial load of the stream water examined during the dry season was 32x102cfu/ml to 56x102 cfu/ml; total coliforms, 14MPN/100ml to 21MPN/100ml and the faecal coliforms 0MPN/100ml. The total bacterial load of the stream during the wet season was 102×102cfu/ml to 160×102cfu/ml; total coliforms, 20MPN/100ml to 43MPN/100ml and the faecal coliforms, 6MPN/100ml to 14MPN/100ml. Shigellae load was 0cfu/ml to 2cfu/ml while Salmonellae, Vibrio cholerae, Enterococcus faecalis and Clostridium perfringens were not detected during both seasons. These results indicated that Iyifeyi stream is unsafe for drinking due to the high bacterial load including the presence of coliforms. Micrococcus varians, Bacillus cereus, Enterobacter aerogenes, Escherichia coli, Shigella dysenteriae and Pseudomonas aeruginosa were detected in the stream water during both seasons. The antibiotics sensitivity test revealed that all the identified bacteria were resistant to Amikacin and Tetracycline. The water from the stream is therefore recommended for treatment such as boiling, sand filtration, chlorination and ozonation before drinking. Copyright@ International Science Community Association