International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 5 2012 09 10 Decolorization of Azo dye Red 3BN by Bacteria 46 52 EN Bhosagar Dullav 1 Department of Environmental Sciences (Auto) Sambalpur University, Jyoti Vihar-768019, Orissa, INDIA BhoiLakshmikanti 2 Mishra P.C. 3 AlitonouGuy Alain 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN Avlessi Felicien 2 Institut des Biomolécules Max Mousseron, IBMM UMR CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE Yehouenou Boniface 3 KoudoroYaya Alain 4 MenutChantalandSohounhloue Dominique 5 DharaskarSwapnilA. 1 Department of Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, MS, INDIA PatilRahul D. 2 Department of Chemical Engineering, Pravara Rural Engineering College, Loni, Ahmednagar, MS, INDIA Joseph Baby 1 nterdisciplinary Research Centre, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA NairVrundhaM 2 Department of Bioinformatics, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA Ch 3 ySusheel G. 4 ParamanathaSwamidoss D. 1 Department of Environmental Science, PSN College of Engg and Tech, Tirunelveli, Tamilnadu, INDIA SudhakaranM.R. 2 Department of Zoology, Sri Paramakalyani College, Alwarkurichi, Tamilnadu, INDIA Parvathiraj P. 3 SharmaP.K. 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, INDIA Ch 2 D. 3 KhelkerTuneer 1 Department of Botany Govt. K.P.G. College Jagdalpur Bastar C.G, PIN 494001 INDIA Haque Nurul 2 PraveenKumarG.N. 1 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA BhatSumangala K. 2 > Research Paper 2012 8 7 2012 09 10 Decolorization of azo dye Red 3BN by two bacterial species Bacillus cereus and B. megaterium has been analyzed using mineral effluent, consisting of known concentration of the dye in ZZ medium. Physico chemical parameters like carbon source, nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition for B. cereus was found to be 1% sucrose 0.25% peptone, pH 7, 37°C and 8% inoculum and that for B.megaterium was found to be glucose 1% , 0.25% yeast extract, pH 6, 37C and 10% inoculum. Extent of decolorization recorded by B. cereus under ideal conditions was 93.64% and that by B.megaterium was 96.88%. The study has confirmed the potential of B. cereus and B. megaterium in the decolorization of Azo dye Red 3BN and opened scope for future analysis of their performance in the treatment of textile effluent. Copyright@ International Science Community Association