International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 5 2012 09 10 Production of cellulase free thermostable xylanase from Pseudomonas sp. XPB-6 31 41 EN Bhosagar Dullav 1 Department of Environmental Sciences (Auto) Sambalpur University, Jyoti Vihar-768019, Orissa, INDIA BhoiLakshmikanti 2 Mishra P.C. 3 AlitonouGuy Alain 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN Avlessi Felicien 2 Institut des Biomolécules Max Mousseron, IBMM UMR CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE Yehouenou Boniface 3 KoudoroYaya Alain 4 MenutChantalandSohounhloue Dominique 5 DharaskarSwapnilA. 1 Department of Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, MS, INDIA PatilRahul D. 2 Department of Chemical Engineering, Pravara Rural Engineering College, Loni, Ahmednagar, MS, INDIA Joseph Baby 1 nterdisciplinary Research Centre, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA NairVrundhaM 2 Department of Bioinformatics, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA Ch 3 ySusheel G. 4 ParamanathaSwamidoss D. 1 Department of Environmental Science, PSN College of Engg and Tech, Tirunelveli, Tamilnadu, INDIA SudhakaranM.R. 2 Department of Zoology, Sri Paramakalyani College, Alwarkurichi, Tamilnadu, INDIA Parvathiraj P. 3 SharmaP.K. 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, INDIA Ch 2 D. 3 > Research Paper 2012 7 8 2012 09 10 Pseudomonas sp. XPB-6 grows better in the medium containing xylan, glucose and meat extract, which was further modified by optimizing the concentrations of individual components. The organism was able to utilize most of the sugars for growth; however dextrose at 4% concentration proved to be the most suitable carbon source for production of xylanase activity from Pseudomonas sp. XPB-6. Different inorganic and organic nitrogen supplements were also tested and maximum production was achieved with 0.5% meat extract. The optimum temperature, pH substrate concentration and inoculum size for the growth and xylanase production were 30C, 7.0, 1% and 6% respectively. The optimum time for growth and enzyme production was 24 hours. Maximum xylanase activity was reported with 100 mM sodium phosphate buffer (pH 7.5) at 55C and 5 minutes incubation temperature. The enzyme was fairly stable at 25\rC and 75\rC. While studying the effect of various metal ions (addition of 1mM CuSO.5HO, CdCl.2HO, ZnSO.7HO and CaCl.2HO) drastic decrease in xylanase activity was observed. Copyright@ International Science Community Association