International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 5 2012 09 10 AmiRzyn: PERL Centered Artificial MicroRNA Designing Aid 18 23 EN Bhosagar Dullav 1 Department of Environmental Sciences (Auto) Sambalpur University, Jyoti Vihar-768019, Orissa, INDIA BhoiLakshmikanti 2 Mishra P.C. 3 AlitonouGuy Alain 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN Avlessi Felicien 2 Institut des Biomolécules Max Mousseron, IBMM UMR CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE Yehouenou Boniface 3 KoudoroYaya Alain 4 MenutChantalandSohounhloue Dominique 5 DharaskarSwapnilA. 1 Department of Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, MS, INDIA PatilRahul D. 2 Department of Chemical Engineering, Pravara Rural Engineering College, Loni, Ahmednagar, MS, INDIA Joseph Baby 1 nterdisciplinary Research Centre, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA NairVrundhaM 2 Department of Bioinformatics, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA Ch 3 ySusheel G. 4 > Research Paper 2012 6 26 2012 09 10 RNA interference, a gene knock down tool has become a promising technology for researchers. Gene silencing, can be fastened by the process of designing small non- coding RNAs through computational tools. One of the current advancement in this field is artificial microRNA. It exploits the backbone of a miRNA for designing siRNA. The endogenous nature of the miRNA provides more success to this technology. In the current study, a tool named Amirzyn was developed for designing artificial microRNA using PERL language, which is very powerful in string manipulations. The tool facilitates the user to input their DNA sequences and by means of pre- defined parameters, the possible siRNA sequences are predicted as output. Possibilities of occurrence of off- target effects are verified by performing BLAST comparison. For amiRNA designing, AmiRzyn permits the specification of restriction site and miRNA backbone of user’s requirement, in addition to the predicted siRNA sequence as the input. AmiRzyn serves as a promising aid for researchers, in the field of gene silencing, to design artificial microRNAs. Copyright@ International Science Community Association