International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 5 2012 09 10 GC-MS, HPTLC and Antimicrobial analysis of Root extracts of Pseudarthria viscida Wight and Arn and Desmodium gangeticum (Linn) DC 57 65 EN Bhosagar Dullav 1 Department of Environmental Sciences (Auto) Sambalpur University, Jyoti Vihar-768019, Orissa, INDIA BhoiLakshmikanti 2 Mishra P.C. 3 AlitonouGuy Alain 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN Avlessi Felicien 2 Institut des Biomolécules Max Mousseron, IBMM UMR CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE Yehouenou Boniface 3 KoudoroYaya Alain 4 MenutChantalandSohounhloue Dominique 5 DharaskarSwapnilA. 1 Department of Chemical Engineering, Visvesvaraya National Institute of Technology, Nagpur, MS, INDIA PatilRahul D. 2 Department of Chemical Engineering, Pravara Rural Engineering College, Loni, Ahmednagar, MS, INDIA Joseph Baby 1 nterdisciplinary Research Centre, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA NairVrundhaM 2 Department of Bioinformatics, Malankara Catholic College, Mariagiri, Kaliakkavilai- 629 153, Tamil Nadu, INDIA Ch 3 ySusheel G. 4 ParamanathaSwamidoss D. 1 Department of Environmental Science, PSN College of Engg and Tech, Tirunelveli, Tamilnadu, INDIA SudhakaranM.R. 2 Department of Zoology, Sri Paramakalyani College, Alwarkurichi, Tamilnadu, INDIA Parvathiraj P. 3 SharmaP.K. 1 Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, INDIA Ch 2 D. 3 KhelkerTuneer 1 Department of Botany Govt. K.P.G. College Jagdalpur Bastar C.G, PIN 494001 INDIA Haque Nurul 2 PraveenKumarG.N. 1 Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA BhatSumangala K. 2 GorhamK. 1 Department of Science and Technology, Bryant University, Smithfield, RI, USA Hokeness K. 2 HemlalH 1 Department of Chemistry, Karpagam University, Eachanari, Coimbatore-641 021, INDIA Subban Ravi 2 > Research Paper 2012 8 14 2012 09 10 The present study was performed to evaluate the chemical composition of the methanol extract of Pseudarthria viscida (L) Wight and Arn and Desmodium gangeticum (Linn) DC. 43 compounds have been identified from P. viscida extract and the major chemical constituents are cis-Vaccenic acid (16.47%), sitosterol (13.73%) and stigmasterol (6.24%).18 compounds have been identified from D.gangeticum and the major chemical constituents are 9,12-Octadecadienoic acid (41.71%), n-Hexadecanoic acid (9.43%) and Octadecanoic acid (5.9%). Stigmasterol was quantified from both extracts by HPTLC method (105.15 µg/ml and 20.9 µg/ml respectively). In- vitro antibacterial and antifungal activities of methanolic extracts of P.viscida and D.gangeticum was evaluated in the present study. The zone of inhibition and minimum inhibitory concentration were measured. Ampicillin (30 g/disc) and mystatin (20 g/disc) were used as standard for antibacterial and antifungal activity respectively. Copyright@ International Science Community Association