International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 4 2012 08 10 Sodium Dodecyl sulphate Polyacrylamide gel Electrophoresis Pattern of Horse Gram Seed Storage Proteins during Germination 39 50 EN Swaminathan C. 1 Tamil Nadu Agricultural University, Agricultural College and Research Institute, Madurai – 625 104, TN, INDIA VijendraRaoR. 2 Wood Properties and Uses division, Institute of Wood Science and Technology, Bengaluru – 560 003, Karnataka, INDIA Shashikala S. 3 Masih Usha 1 Department of Botany, Mata Jija Bai Govt. Girls P.G. College, Indore, MP, INDIA ShrimaliRagini 2 Department of Microbiology, I.K. Science College Indore, MP, INDIA Naqvi S.M.A 3 Department of Botany, I. K. Science College Indore, MP, INDIA Mehta Jitendra 1 Microbiology and Pathology Division, Vital Biotech Research Institute, University of Kota, Kota- 324009, Rajasthan, INDIA DhakerJayraj Kiran 2 Kavia Ambika 3 Sen Priya 4 KaushalNeha 5 Datta Sakshi 6 QuadrosGoldin 1 B.N. Bandodkar College of Science, Chendani, Thane 400601, Maharashtra, INDIA Athalye R.P. 2 álim Ali Centre for Ornithology and Natural History (SACON), Anaikatti Post, Coimbatore 641108, Tamil Nadu, INDIA Philippe Sessou 1 Laboratoire de Recherche en Biologie Appliquée (LARBA), Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, BENIN Souaïbou Farougou 2 Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey-Calavi (EPAC), Cotonou, BENIN Guy Alitonou 3 Laboratoire de Microbiologie et de Biotechnologie Alimentaire, Faculté des Sciences Agronomiques (FSA), Cotonou, BENIN Sébastien Djenontin Tindo 4 Boniface Yèhouénou 5 Paulin Azokpota 6 Issaka Youssao 7 Dominique Sohounhloué 8 SaralaThambavaniD. 1 Department of Chemistry, Sri Meenakshi Government Arts College for Women (Autonomous), Madurai-625, INDIA Sabitha M.A. 2 Department of Chemistry, J.A. College for Women (Autonomous), Periyakulam-625 601, INDIA IllathSujina 1 * Department of Chemistry, Karpagam University, Coimbatore-641 021, INDIA Subban Ravi 2 PekGeok Pang 1 Faculty of Agro Based Industry, University Malaysia Kelantan (Jeli campus), Beg Berkunci No. 100, 17600 Jeli, Kelantan, MALAYSIA Asrul Af 2 Faculty of Earth science, University Malaysia Kelantan (Jeli campus), Beg Berkunci No. 100, 17600 Jeli, Kelantan, MALAYSIA i 3 Faculty of Agro Based Industry, University Malaysia Kelantan (Jeli campus), Beg Berkunci No. 100, 17600 Jeli, Kelantan, MALAYSIA RahmanShefiqur 4 ShahaRanajit kumar 5 > Research Paper 2012 7 10 2012 08 10 The aim of the experiment was to study the effect of germination on the three varieties of horse gram seed storage proteins. Seeds were germinated for 24, 48, 72, 96 and 110h in a dark place. Germinated seeds were frozen at -18C for 12h to stop the germination process. Spouted seeds were freeze-dried and ground to pass through a 40 mesh sieve for analysis. Total proteins, water soluble and water insoluble proteins were estimated by standard methods. Seed storage protein profiles of three varieties germinated horse gram were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Extracted protein fractions from germinated horse gram seeds in different solvents were studied by HPLC. The highest amount of storage protein degradation was observed in all varieties after 72-80h of germination. Characterization of protein fractions by HPLC showed that albumins/globulins, prolamins and glutelins increases slightly during germination time, as germinating seeds usually accompany by interconversion and production of new compounds. Electrophoregrams for each variety were shown and the high molecular weight proteins band intensity decreases during different germination period were noted. Genetic diversity of germinated horse gram was evaluated by constructing the dendrogram for high molecular weight (HMW) and low molecular weight (LMW) gluten subunit bands. In conclusion, SDS-PAGE of germinated seed storage proteins can be economically used to assess genetic variation and relation in germplasm. The specific bands of germinated seed storage protein profiles may be used as markers for identification of the mutants/genotypes Copyright@ International Science Community Association