International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 4 2012 08 10 In-vitro Antimicrobial and Cytotoxic ativity of Methanolic extract of Osbeckia wynaadensis 33 38 EN Swaminathan C. 1 Tamil Nadu Agricultural University, Agricultural College and Research Institute, Madurai – 625 104, TN, INDIA VijendraRaoR. 2 Wood Properties and Uses division, Institute of Wood Science and Technology, Bengaluru – 560 003, Karnataka, INDIA Shashikala S. 3 Masih Usha 1 Department of Botany, Mata Jija Bai Govt. Girls P.G. College, Indore, MP, INDIA ShrimaliRagini 2 Department of Microbiology, I.K. Science College Indore, MP, INDIA Naqvi S.M.A 3 Department of Botany, I. K. Science College Indore, MP, INDIA Mehta Jitendra 1 Microbiology and Pathology Division, Vital Biotech Research Institute, University of Kota, Kota- 324009, Rajasthan, INDIA DhakerJayraj Kiran 2 Kavia Ambika 3 Sen Priya 4 KaushalNeha 5 Datta Sakshi 6 QuadrosGoldin 1 B.N. Bandodkar College of Science, Chendani, Thane 400601, Maharashtra, INDIA Athalye R.P. 2 álim Ali Centre for Ornithology and Natural History (SACON), Anaikatti Post, Coimbatore 641108, Tamil Nadu, INDIA Philippe Sessou 1 Laboratoire de Recherche en Biologie Appliquée (LARBA), Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou, BENIN Souaïbou Farougou 2 Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey-Calavi (EPAC), Cotonou, BENIN Guy Alitonou 3 Laboratoire de Microbiologie et de Biotechnologie Alimentaire, Faculté des Sciences Agronomiques (FSA), Cotonou, BENIN Sébastien Djenontin Tindo 4 Boniface Yèhouénou 5 Paulin Azokpota 6 Issaka Youssao 7 Dominique Sohounhloué 8 SaralaThambavaniD. 1 Department of Chemistry, Sri Meenakshi Government Arts College for Women (Autonomous), Madurai-625, INDIA Sabitha M.A. 2 Department of Chemistry, J.A. College for Women (Autonomous), Periyakulam-625 601, INDIA IllathSujina 1 * Department of Chemistry, Karpagam University, Coimbatore-641 021, INDIA Subban Ravi 2 > Research Paper 2012 7 6 2012 08 10 In- vitro antibacterial and antifungal activities of methanolic extract of osbeckia wynaadensis was evaluated in the present study for the first time by disc diffusion method using five bacterial strains (S. pneumonia, B.cereus, A. hydrophila , V. cholera and MRSA) and five fungal strains (Candida albicans, Aspergillus niger, Streptomysis Greusis, M. purpureus and Aspergillus fumigate) respectively. The zone of inhibition and minimum inhibitory concentration were measured. Ampicillin (30 g/disc) and clotrimazole (20 g/disc) were used as standard for antibacterial and antifungal activity respectively. In-vitro cytotoxic activity of Osbeckia wynaadensis was evaluated against the human cervical adenocarcinoma cell line (HeLa) and murine embryonic fibroblasts cell line (NIH 3T3) by MTT assay and the IC50 value found to be 220.3 g/mL and 93. 25 g/mL. It is concluded that Osbeckia wynaadensis exhibited significant antibacterial, antifungal & in-vitro cytotoxic activity. Copyright@ International Science Community Association