International Science Community Association International Research Journal of Biological Sciences 2278 - 3202 1 3 2012 07 10 Purification of a Novel Thermophilic Lipase from B. Licheniformis MTCC-10498 43 48 EN Patel AnuradhaJitendra 1 Department of Zoology, Bhavans College, Andheri, Mumbai University, Mumbai, INDIA Thakor AkankshaPravin 2 Department of Biotechnology, N.B. Mehta Science College, Bordi, Mumbai University, Mumbai, INDIA Avlessi Félicien 1 Lab. d’Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d’Abomey-Calavi, Université d’Abomey-Calavi, Cotonou BENIN AlitonouGuy Alain 2 Institut des Biomolécules Max Mousseron, IBMM UMR, CNRS-UM1-UM2, ENSCM, rue de l’Ecole Normale, Montpellier cedex, FRANCE DjenontinT. Sébastien 3 Tchobo Fidele 4 Yèhouénou Boniface 5 MenutChantal 6 Sohounhloué Dominique 7 Radha K. 1 Department of Microbiology, Ayya Nadar Janaki ammal College, Sivakasi, Tamilnadu, INDIA Mahima R. 2 Department of Microbiology, V.H.N.S.N College, Virudhunagar, Tamilnadu, INDIA RamanathanG. 3 Thangap 4 ian V. 5 Bhatt T.K. 1 Department of Biotechnology, Central University of Rajasthan, Kishangarh, INDIA DjenontinTindo S. 1 1Laboratoire d’Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d’Abomey – Calavi (EPAC), Cotonou, BENIN Amusant N. 2 CIRAD, UMR 016 GPEB, Montpellier, FRANCE Dangou J. 3 CIRAD, UMR ECOFOG, Equipe Matériaux et Molécules, Kourou, FRANCE Wotto D.V. 4 CIRAD, UR 40 Tropical Wood Products, Biorefinery team, Montpellier, FRANCE Avlessi F. 5 Dahouénon-Ahoussi E. 6 Lozano P. 7 PiochD. 8 Sohounhloué K.C.D. 9 Majumdar Tania 1 Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata-700032, INDIA ChakrabortyRunu 2 Raychaudhuri Utpal 3 Mehta Jitendra 1 Plant Tissue Culture Lab. and Dept. of Biotech., Vital Biotech Research Inst., University of Kota, Kota-Rajasthan, INDIA Sain Monika 2 SharmaDev Ratan 3 Gehlot Priyanka 4 SharmaPriyanka 5 DhakerJayraj Kiran 6 Sharma Ch 1 Department of Biotechnology, Himachal Pradesh University, Shimla-171005, INDIA erK. 2 KanwarShamsher S. 3 > Research Paper 2012 6 11 2012 07 10 Lipases have emerged as one of the leading biocatalysts with proven potential for contributing to the multibillion dollar underexploited lipid technology bio-industry and have been used in situ lipid metabolism and ex situ multifaceted industrial applications. For certain applications, such as synthetic reactions in pharmaceutical industry, further purification is needed. Since lipases are known to be hydrophobic in nature, having large hydrophobic surfaces around the active site, the purification of lipases may best be achieved by opting for affinity chromatography, such as hydrophobic interaction chromatography. The purification of bacterial lipase was performed using techniques of ammonium sulphate salting out, dialysis and hydrophobic interaction chromatography (Octyl sepharose) respectively. The analysis of bacterial lipase under reducing and denaturing SDS- PAGE (polyacrylamide gel electrophoresis) revealed that the purified lipase possessed a single band of MW 19kDa as visualized with Coomasie Brilliant Blue R-250. Copyright@ International Science Community Association